The sequence of bases in a DNA template strand is 5'GGCTTATTGCCA3'. What is the corresponding mRNA produced?

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Identify the direction of the DNA template strand. The given strand is 5' to 3', so transcription will occur in the 3' to 5' direction.

Recall the base-pairing rules for transcription: Adenine (A) pairs with Uracil (U), Thymine (T) pairs with Adenine (A), Cytosine (C) pairs with Guanine (G), and Guanine (G) pairs with Cytosine (C). Note that Uracil (U) replaces Thymine (T) in RNA.

Write the complementary mRNA strand by pairing each base of the DNA template strand with its corresponding RNA base, starting from the 3' end of the DNA template strand.

Reverse the sequence of the complementary mRNA strand to ensure it is written in the 5' to 3' direction, as mRNA is synthesized in this orientation.

Verify the final mRNA sequence by double-checking the base-pairing and orientation to ensure accuracy.

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DNA Structure

DNA is composed of two strands forming a double helix, with each strand made up of nucleotides. Each nucleotide consists of a sugar, a phosphate group, and a nitrogenous base (adenine, thymine, cytosine, or guanine). The sequence of these bases encodes genetic information, and the directionality of the strands is indicated as 5' to 3'.

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3:27

DNA Double Helix Concept 1

Transcription

Transcription is the process by which the genetic information in DNA is copied into messenger RNA (mRNA). During transcription, RNA polymerase reads the DNA template strand and synthesizes a complementary RNA strand, replacing thymine (T) with uracil (U). This process is essential for gene expression and occurs in the nucleus of eukaryotic cells.

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6:37

Transcription: mRNA Synthesis Concept 1

Base Pairing Rules

Base pairing rules dictate how nucleotides pair during DNA replication and transcription. In DNA, adenine pairs with thymine, and cytosine pairs with guanine. During transcription, the base pairing changes slightly, as adenine pairs with uracil in RNA. Understanding these rules is crucial for determining the correct mRNA sequence from a given DNA template.

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