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Conjugation: F Plasmids quiz

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  • What is a conjugative plasmid?
    A conjugative plasmid is a plasmid capable of directing its own transfer to a recipient cell via conjugation.
  • What does the 'F' in F plasmid stand for?
    The 'F' in F plasmid stands for fertility.
  • Which type of cell contains the entire F plasmid?
    F+ cells contain the entire F plasmid.
  • What structure do F+ cells synthesize to initiate conjugation?
    F+ cells synthesize an F pilus to initiate conjugation.
  • What is the role of F- cells in conjugation?
    F- cells serve as recipient cells in conjugation because they lack the entire F plasmid.
  • Can F- cells initiate conjugation? Why or why not?
    No, F- cells cannot initiate conjugation because they do not have an F pilus.
  • What is the tra region on the F plasmid responsible for?
    The tra region contains genes required for conjugation, including those that create the F pilus.
  • What is the function of the oriT region on the F plasmid?
    The oriT region serves as the origin of replication during conjugation.
  • Describe the first step in F plasmid conjugation.
    The F pilus of the F+ donor cell binds to a receptor on the outer membrane of the F- recipient cell.
  • What happens after the F pilus binds to the F- cell?
    The F pilus retracts, pulling the two cells closer together.
  • At what region is the F plasmid cut during conjugation?
    The F plasmid is cut at the oriT region.
  • What is transferred from the F+ cell to the F- cell during conjugation?
    A single strand of the F plasmid is transferred from the F+ cell to the F- cell.
  • How does the recipient cell become F+ after conjugation?
    The single strand of the F plasmid is replicated in the recipient cell, forming a complete F plasmid and converting it to F+.
  • What is the outcome for both cells after F plasmid conjugation is complete?
    Both the donor and recipient cells become F+ cells, each with a full copy of the F plasmid.
  • Why is the spread of the F plasmid significant in bacterial populations?
    The spread of the F plasmid allows more cells to become F+ and capable of further conjugation, facilitating genetic exchange.