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Indietro

Exam 1

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  • What is metabolism in microbes?

    Metabolism is the collection of controlled biochemical reactions within a microbe, ultimately functioning to reproduce the organism.

  • What are the two main types of metabolic pathways?

    Catabolic pathways break down molecules and release energy (exergonic). Anabolic pathways synthesize molecules and require energy (endergonic).

  • What is the primary energy currency in microbial metabolism?

    ATP (adenosine triphosphate) stores and transfers energy within cells.

  • What is glycolysis and where does it occur?

    Glycolysis is the splitting of glucose into two pyruvic acid molecules, occurring in the cytoplasm of most cells.

  • What is substrate-level phosphorylation?

    Direct transfer of a phosphate group between substrates to form ATP during glycolysis and Krebs cycle.

  • What are the three stages of glycolysis?

    Energy-investment stage, lysis stage, and energy-conserving stage.

  • What happens during the energy-investment stage of glycolysis?

    Glucose is phosphorylated twice by ATP to form fructose 1,6-bisphosphate.

  • What is the net gain of ATP and NADH from glycolysis?

    2 ATP molecules and 2 NADH molecules per glucose molecule.

  • What is the role of the Pyruvate Dehydrogenase complex?

    Decarboxylates pyruvic acid to acetate, produces CO2 and NADH, and forms acetyl-CoA with a high-energy bond.

  • Where does the Krebs cycle occur and what is its main function?

    Occurs in the cytosol of prokaryotes and mitochondrial matrix in eukaryotes; fully oxidizes acetyl-CoA to CO2 and transfers energy to NADH and FADH2.

  • What are the main types of reactions in the Krebs cycle?

    Anabolism of citric acid, isomerization, decarboxylation, redox reactions, substrate-level phosphorylation, and hydration.

  • How many ATP, NADH, FADH2, and CO2 molecules are produced per glucose in the Krebs cycle?

    2 ATP, 6 NADH, 2 FADH2, and 4 CO2 molecules.

  • What is the electron transport chain (ETC)?

    A series of membrane-bound carriers that transfer electrons to a final acceptor, pumping protons to create a gradient for ATP synthesis.

  • What is the final electron acceptor in aerobic respiration?

    Oxygen, which is reduced to water.

  • How is ATP generated in the ETC?

    Proton gradient drives ATP synthase to phosphorylate ADP to ATP, a process called oxidative phosphorylation.

  • How many ATP molecules are produced per glucose via cellular respiration?

    Approximately 38 ATP in prokaryotes and 36 ATP in eukaryotes.

  • What are alternative glycolytic pathways in microbes?

    Entner-Doudoroff pathway and Pentose Phosphate pathway, producing different precursor metabolites and NADPH.

  • What is fermentation and when does it occur?

    Partial oxidation of sugars to regenerate NAD+ when respiration is not possible, producing organic waste products.

  • How does fermentation differ from anaerobic respiration?

    Fermentation uses organic molecules as final electron acceptors and produces less ATP; anaerobic respiration uses inorganic molecules other than oxygen.

  • How are lipids catabolized in microbes?

    Lipids are hydrolyzed into glycerol and fatty acids; glycerol enters glycolysis, fatty acids undergo beta-oxidation to form acetyl-CoA.

  • What is the formula to calculate ATP yield from fatty acid beta-oxidation?

    \((\frac{N}{2}-1) \times 5 + (\frac{N}{2} \times 12)\), where N is the number of carbons.

  • How are proteins catabolized in microbes?

    Proteins are broken down extracellularly into amino acids, transported into the cell, and deaminated to enter the Krebs cycle.

  • What are anabolic reactions in microbial metabolism?

    Synthesis of carbohydrates, lipids, amino acids, and nucleotides using energy from ATP and precursor metabolites.

  • What is gluconeogenesis?

    Synthesis of sugars from non-carbohydrate precursors like fats and proteins, often reversing glycolysis steps.

  • How do microbes regulate metabolic pathways?

    By controlling enzyme production (gene expression) and enzyme activity (allosteric regulation, feedback inhibition).