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Introduction to Microbiology

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  • What is microbiology?

    Microbiology is the study of microorganisms or microbes, which are often invisible to the naked eye.

  • What types of organisms are included in the term 'microbe'?

    Microbes include cellular living microorganisms like bacteria, archaea, fungi, protists, helminths, and nonliving entities such as viruses and prions.

  • What distinguishes prokaryotic from eukaryotic cells?

    Prokaryotic cells lack a nucleus and include bacteria and archaea. Eukaryotic cells have a nucleus and include fungi, protists, and multicellular organisms.

  • What is the difference between a pathogen and an opportunistic pathogen?

    Pathogens always cause disease in humans, while opportunistic pathogens cause disease only in weakened hosts.

  • What is spontaneous generation versus biogenesis?

    Spontaneous generation is the disproven theory that life arises from nonliving matter. Biogenesis states life arises from existing life.

  • Who disproved spontaneous generation and how?

    Louis Pasteur disproved spontaneous generation using an S-necked flask experiment showing that air contains microbes but they do not spontaneously generate.

  • What is the germ theory of disease?

    The germ theory of disease states that microbes cause infectious diseases.

  • What are Koch's postulates?

    Four criteria to link a microbe to a disease: 1) Present in every case, 2) Isolated and grown in pure culture, 3) Causes disease in a healthy host, 4) Re-isolated from the infected host.

  • What contributions did Semmelweis, Lister, and Nightingale make to healthcare?

    Semmelweis promoted hand washing to reduce infections, Lister introduced aseptic surgery techniques, and Nightingale established aseptic nursing practices.

  • What is aseptic technique and why is it important?

    Aseptic technique prevents contamination and healthcare-acquired infections by practices like hand washing, sterilizing instruments, and decontaminating surfaces.

  • What is the scientific method in microbiology?

    It involves asking a question, proposing a hypothesis, collecting data through observation, and drawing conclusions to support or refute the hypothesis.

  • What is the difference between an observation and a conclusion?

    An observation is data collected using senses or instruments; a conclusion interprets observations to explain findings.

  • How do scientific laws differ from scientific theories?

    Laws predict what happens, often as precise statements or formulas; theories explain how and why phenomena occur, supported by extensive evidence.

  • What is taxonomy in microbiology?

    Taxonomy is the classification of organisms based on shared features, including morphology and physiology.

  • What is the taxonomic hierarchy from broadest to most specific?

    Domain, Kingdom, Phylum, Class, Order, Family, Genus, Species.

  • What are the three domains of life?

    Bacteria (unicellular prokaryotes), Archaea (extremophilic prokaryotes, no known pathogens), and Eukarya (unicellular and multicellular eukaryotes).

  • What is binomial nomenclature?

    A two-name system for naming organisms: the genus name (capitalized) and species name (lowercase), both italicized, e.g., Escherichia coli.

  • What is normal microbiota?

    Microbes that normally live on or in the human body, including bacteria, archaea, and eukaryotic microbes, which help train the immune system and aid digestion.

  • How is normal microbiota established in humans?

    Microbes colonize babies during delivery and early life through environmental exposure, influenced by delivery mode and feeding type.

  • What happens when normal microbiota is disrupted?

    Disruption, such as by antibiotics, can reduce protective microbes and allow opportunistic pathogens to cause infections.

  • What are transient microbiota?

    Temporary microbes acquired from the environment that do not persist and can be removed by hygiene practices like hand washing.