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Microbiology: Biochemistry Basics

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  • What is an atom?

    An atom is the smallest unit of an element, consisting of a nucleus with protons and neutrons, surrounded by electrons.
  • What are the charges of subatomic particles?

    Protons are positively charged, neutrons are neutral, and electrons are negatively charged.
  • What defines an element's atomic number?

    The atomic number equals the number of protons in an atom and uniquely identifies the element.
  • What is an ion?

    An ion is a charged atom with an unequal number of protons and electrons.
  • Difference between cation and anion?

    Cations are positively charged ions (lost electrons), anions are negatively charged ions (gained electrons).
  • What are isotopes?

    Isotopes are atoms of the same element with the same number of protons but different numbers of neutrons.
  • Define molecule and compound.

    A molecule is two or more atoms bonded together; a compound is a molecule made of different elements.
  • What is a molecular formula?

    A molecular formula shows the types and numbers of atoms in a molecule.
  • Difference between organic and inorganic molecules?

    Organic molecules contain carbon and hydrogen; inorganic molecules may lack hydrogen.
  • What are functional groups?

    Functional groups are specific groups of atoms within molecules that determine chemical properties and reactions.
  • Name key biologically important functional groups.

    Alcohol (R-OH), Amine (R-NH2), Carboxyl (R-COOH), Ester (R-COO-R'), Methyl (R-CH3), Phosphate (R-PO4^2-), Sulfhydryl (R-SH).
  • What is an acid and a base in aqueous solutions?

    Acids add hydrogen ions (H+) to solution; bases add hydroxide ions (OH-).
  • What is a salt in chemistry?

    Salts form when acids and bases react, combining anions from acids and cations from bases.
  • What does the pH scale measure?

    The pH scale measures the acidity or basicity of a solution based on hydrogen ion concentration.
  • What is a buffer?

    A buffer stabilizes pH by absorbing or releasing hydrogen ions to maintain a constant pH.
  • What are valence electrons?

    Valence electrons are electrons in the outermost shell that participate in chemical bonding.
  • Difference between ionic and covalent bonds?

    Ionic bonds form by transfer of electrons creating charged ions; covalent bonds form by sharing electron pairs.
  • What is a polar covalent bond?

    A polar covalent bond involves unequal sharing of electrons, creating partial positive and negative charges.
  • What are hydrogen bonds?

    Hydrogen bonds are weak electrostatic attractions between polar molecules or within large molecules.
  • Define hydrophilic, hydrophobic, and amphipathic molecules.

    Hydrophilic molecules dissolve in water; hydrophobic molecules repel water; amphipathic molecules have both properties.
  • What is a synthesis reaction?

    A synthesis reaction builds larger molecules from smaller reactants, often releasing water (dehydration synthesis).
  • What is a decomposition reaction?

    A decomposition reaction breaks down molecules into smaller components, often adding water (hydrolysis).
  • What is activation energy?

    Activation energy is the minimum energy required to start a chemical reaction.
  • Difference between endergonic and exergonic reactions?

    Endergonic reactions require more energy than they release; exergonic reactions release more energy than they require.
  • What are the four main classes of biomolecules?

    Carbohydrates, lipids, nucleic acids, and proteins.
  • What are the building blocks of carbohydrates?

    Simple sugars called monosaccharides.
  • What type of bond links monosaccharides?

    Glycosidic bonds formed by dehydration synthesis.
  • Difference between saturated and unsaturated fats?

    Saturated fats have no double bonds and are solid at room temperature; unsaturated fats have double bonds and are liquid.
  • What are nucleotides composed of?

    A five-carbon sugar, one or more phosphate groups, and a nitrogenous base.
  • What is the difference between DNA and RNA nucleotides?

    DNA contains deoxyribose sugar and thymine; RNA contains ribose sugar and uracil.
  • What are proteins made of?

    Proteins are polymers of amino acids linked by peptide bonds.
  • Name the four levels of protein structure.

    Primary (amino acid sequence), secondary (alpha-helices and beta-sheets), tertiary (3D folding), quaternary (multiple polypeptide chains).
  • What role do chaperone proteins play?

    Chaperones assist in proper protein folding to achieve functional structure.