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Microbiology Module 1 Study Guide Flashcards

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  • What is the theory of spontaneous generation?

    The idea that living organisms can arise from nonliving matter spontaneously without parent organisms.

  • What scientific evidence disproved spontaneous generation?

    Experiments by Louis Pasteur using swan-neck flasks showed that microorganisms come from other microorganisms, supporting biogenesis.

  • Contributions of Louis Pasteur to microbiology

    Disproved spontaneous generation, developed pasteurization, and contributed to germ theory of disease.

  • What did Robert Koch contribute to microbiology?

    Developed Koch's postulates to link specific microbes to specific diseases and advanced pure culture techniques.

  • Role of Joseph Lister in microbiology

    Introduced antiseptic surgical techniques to reduce infections using carbolic acid (phenol).

  • Ignaz Semmelweis's contribution to microbiology

    Advocated handwashing in medical settings to prevent puerperal fever and reduce infections.

  • Who was Robert Hooke and his contribution?

    First to describe cells in cork, coining the term 'cell' in biology.

  • Antonie van Leeuwenhoek's role in microbiology

    First to observe and describe microorganisms using handcrafted microscopes.

  • Florence Nightingale's impact on microbiology and health

    Used statistical data to improve sanitary conditions in hospitals, reducing infections and mortality.

  • Common features of living things

    Growth, reproduction, metabolism, cellular organization, response to stimuli, and homeostasis.

  • Distinguishing features of bacteria

    Prokaryotic, unicellular, cell walls with peptidoglycan, reproduce by binary fission.

  • Distinguishing features of fungi

    Eukaryotic, can be unicellular or multicellular, cell walls made of chitin, reproduce via spores.

  • What is a mycosis?

    A fungal infection affecting humans or animals.

  • Difference between bacterial endospores and fungal spores

    Endospores are dormant, resistant structures for survival; fungal spores are reproductive units for dispersal.

  • Why are bacterial endospores challenging in healthcare?

    They resist heat, chemicals, and disinfectants, making sterilization difficult.

  • How are protozoans classified?

    Based on locomotion: flagella, cilia, pseudopodia, or non-motile forms.

  • Two main groups of parasitic helminths

    Nematodes (roundworms) and platyhelminths (flatworms, including flukes and tapeworms).

  • Define total magnification in microscopy

    Product of the ocular lens magnification and the objective lens magnification.

  • What is resolution in microscopy?

    The ability to distinguish two close objects as separate entities.

  • Purpose of oil in oil immersion microscopy

    Increases resolution by reducing light refraction between the slide and lens.

  • Difference between transmission electron microscopy (TEM) and scanning electron microscopy (SEM)

    TEM shows internal structures by transmitting electrons; SEM shows surface details by scanning electrons.

  • What information does a Gram stain provide?

    Classifies bacteria as Gram-positive or Gram-negative based on cell wall properties.

  • Two acid-fast genera and why they are acid-fast

    Mycobacterium and Nocardia; they have waxy mycolic acid in their cell walls resisting decolorization.

  • Define microbiome

    The community of microorganisms living in or on a host organism, often beneficial or neutral.

  • Describe parasitism

    A symbiotic relationship where one organism benefits and the other is harmed.

  • Describe mutualism

    A symbiotic relationship where both organisms benefit.

  • Describe commensalism

    A symbiotic relationship where one organism benefits and the other is unaffected.

  • What is a biofilm?

    A community of microorganisms attached to a surface, embedded in a protective matrix.

  • Why are biofilms important in healthcare?

    They protect microbes from antibiotics and immune responses, causing persistent infections.

  • Examples of beneficial microbial roles in industry

    Bioremediation, food and beverage production, drug manufacturing, and biofuel production.

  • What is bioremediation?

    Use of microbes to clean up environmental pollutants.

  • Difference between dehydration synthesis and hydrolysis

    Dehydration synthesis builds molecules by removing water; hydrolysis breaks molecules by adding water.

  • Difference between covalent, ionic, and hydrogen bonds

    Covalent bonds share electrons, ionic bonds transfer electrons, hydrogen bonds are weak attractions between polar molecules.

  • Four major macromolecule groups and their monomers

    Carbohydrates (monosaccharides), lipids (glycerol and fatty acids), proteins (amino acids), nucleic acids (nucleotides).

  • Basic lab safety PPE in microbiology

    Gloves, lab coat, eye protection, and closed-toe shoes.

  • Proper disposal of petri plates with bacterial cultures

    Autoclave or use biohazard disposal methods to prevent contamination.

  • How to calculate total magnification

    Multiply ocular lens magnification by objective lens magnification (e.g., 10x × 40x = 400x).