Skip to main content
Ch. 14 - Biomedical Applications: Vaccines, Diagnostics, Therapeutics, and Molecular Method
Norman-McKay- Microbiology: Basic and Clinical Principles 2nd Edition
Norman-McKay2nd EditionMicrobiology: Basic and Clinical PrinciplesISBN: 9780137661619Non è quello che usi tu?Cambia libro di testo
Tutti i libri di testoNorman-McKay 2nd EditionCh. 14 - Biomedical Applications: Vaccines, Diagnostics, Therapeutics, and Molecular MethodProblema 14
Capitolo 10, Problema 14

How many copies could PCR make if we started with one copy of a template and performed 15 cycles of copying?
a. A few
b. A few hundred
c. A few thousand
d. Millions

Guida verificata passo dopo passo
1
Understand that PCR (Polymerase Chain Reaction) amplifies DNA by doubling the number of copies each cycle, assuming 100% efficiency.
Recall the formula for the number of DNA copies after n cycles starting from one copy: \(\text{Number of copies} = 2^{n}\), where \(n\) is the number of cycles.
Substitute the given number of cycles (15) into the formula: \(\text{Number of copies} = 2^{15}\).
Calculate or estimate the value of \$2^{15}$ to determine the approximate number of copies produced after 15 cycles.
Compare the calculated number to the options provided (a few, a few hundred, a few thousand, millions) to select the correct answer.

Risposta video verificata per un problema simile:

Questa soluzione video è stata consigliata dai nostri tutor come utile per risolvere questo problema.
Durata del video:
2m

Concetti chiave

Ecco i concetti essenziali che devi comprendere per rispondere correttamente alla domanda.

Polymerase Chain Reaction (PCR) Basics

PCR is a technique used to amplify a specific DNA segment exponentially by cycling through denaturation, annealing, and extension steps. Each cycle ideally doubles the number of DNA copies, leading to rapid accumulation of the target sequence.
Video consigliato:
01:31
Introduction to Polymerase Chain Reaction

Exponential Amplification in PCR

In PCR, the number of DNA copies doubles with each cycle, so after n cycles, the total copies equal 2^n. Starting with one copy, 15 cycles would produce 2^15 copies, illustrating the power of exponential growth in molecular biology.
Video consigliato:
04:39
The Steps of PCR

Calculating PCR Product Quantity

To estimate the number of DNA copies after PCR, use the formula 2^n, where n is the number of cycles. For 15 cycles, 2^15 equals 32,768 copies, which corresponds to tens of thousands, helping to choose the correct answer from the options.
Video consigliato:
04:39
The Steps of PCR
Pratica correlata
Domanda del libro di testo

Place the following steps in order for a neutralization test:

a. Incubate cell culture for a few days

b. Add suspected infecting virus to the patient's serum

c. Inspect cell culture for viral infection

d. Extract serum from patient

e. Mix patient serum with virus and add the mixture to a cell culture

1414
views
Domanda del libro di testo

How could a CRISPR-Cas9 enter a human cell for genome editing purposes?

1612
views
Domanda del libro di testo

Select the true statements about mRNA vaccines. Select all that apply.

a. These vaccines contain a type of nucleic acid

b. These vaccines can alter cellular DNA

c. These vaccines often require a booster dose

d. These vaccines do not require adjuvants

e. These vaccines require an engineered virus to deliver the mRNA to a target cell

688
views
Domanda del libro di testo

Describe one difference between a direct ELISA and an indirect ELISA.

1934
views