Anatomy & Physiology: Cell Biology, Tissues, and Molecular Processes
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DNA
DNA is the molecule that stores genetic information in cells. It consists of nucleotide sequences forming double-stranded helices and serves as the template for transcription.
mRNA
mRNA (messenger RNA) is a single-stranded RNA that carries the genetic code transcribed from DNA to ribosomes for protein synthesis.
tRNA
tRNA (transfer RNA) brings specific amino acids to the ribosome during translation by matching its anticodon to mRNA codons.
DNA triplets, mRNA codons, and tRNA anticodons
A DNA triplet is a sequence of three DNA bases; it is transcribed into an mRNA codon (three RNA bases), which is then matched by a complementary tRNA anticodon during translation.
Helicase
Helicase unwinds the DNA double helix during transcription, separating the strands to allow RNA polymerase access.
RNA polymerase
RNA polymerase synthesizes mRNA by reading the DNA template strand during transcription.
Promotor
A promotor is a DNA sequence where RNA polymerase binds to initiate transcription.
Start codon
The start codon (usually AUG) signals the beginning of translation and codes for the first amino acid, methionine.
Stop codon
A stop codon signals the end of translation, instructing the ribosome to release the completed polypeptide.
Spliceosomes
Spliceosomes are complexes that remove introns from pre-mRNA, joining exons to form mature mRNA.
Introns and exons
Introns are non-coding sequences removed from pre-mRNA; exons are coding sequences joined to form the final mRNA.
Ribosome A, P, and E sites
The A site binds incoming tRNA, the P site holds the tRNA with the growing polypeptide, and the E site is where tRNA exits after delivering its amino acid.
Polypeptide chain formation
Amino acids are joined by dehydration synthesis at the ribosome, forming peptide bonds to create a polypeptide chain.
Golgi apparatus role in translation
The Golgi apparatus modifies, folds, and packages proteins made by ribosomes for export or use within the cell.
Nucleotide and nitrogenous base
A nucleotide is the building block of DNA/RNA, consisting of a sugar, phosphate, and a nitrogenous base (A, T/U, C, G).
Structural differences between DNA and mRNA
DNA is double-stranded with deoxyribose sugar; mRNA is single-stranded with ribose sugar and uracil instead of thymine.
Functional uses of DNA vs RNA types
DNA stores genetic info; mRNA carries code to ribosomes; rRNA forms ribosome structure; tRNA delivers amino acids.
Relationship between amino acid and protein
A protein is a chain of amino acids linked by peptide bonds, folded into a functional molecule.
Gene and genome
A gene is a DNA segment coding for a protein; the genome is the complete set of genes in an organism.
Cytoplasm vs cytosol
Cytoplasm includes cytosol plus organelles; cytosol is the gel-like fluid inside the cell.
Simple squamous epithelium
Flat cells with thin nuclei; found in lung alveoli and capillaries for diffusion of gases and nutrients.
Simple cuboidal epithelium
Cube-shaped cells with round nuclei; found in glands and kidney tubules for secretion and absorption.
Simple columnar epithelium
Tall, column-shaped cells with oval nuclei; found in intestines for nutrient absorption and mucus secretion.
Transitional epithelium
Layered cells that stretch from many layers to one; found in urinary bladder to accommodate volume changes.
Keratinized vs non-keratinized stratified squamous epithelium
Keratinized has dead, tough outer layers (skin); non-keratinized has living surface cells (mouth, esophagus).
Dense fibrous regular connective tissue
Parallel collagen fibers; forms tendons and ligaments providing strong, unidirectional tensile strength.
Skeletal muscle tissue
Striated, multinucleated cells under voluntary control; attached to bones for movement.
Cardiac muscle tissue
Striated, branched cells with intercalated discs; involuntary control for heart contractions.
Smooth muscle tissue
Spindle-shaped, non-striated cells with gap junctions; involuntary control in hollow organs for movement of contents.
Cell membrane integral proteins
Proteins spanning the membrane acting as channels, carriers, receptors, or enzymes for cell communication and transport.