Cell theory states that a cell is the structural and functional unit of life, all living cells arise from pre-existing cells, and all organisms are made of one or more cells.
What are the three basic parts of a generalized human cell?
The three basic parts are: 1) Plasma membrane (flexible outer boundary), 2) Cytoplasm (intracellular fluid with organelles), and 3) Nucleus (DNA-containing control center).
Describe the structure of the plasma membrane.
The plasma membrane is a fluid mosaic model consisting of a phospholipid bilayer with polar heads facing water and nonpolar tails inside, with floating integral and peripheral proteins.
What are the functions of membrane proteins?
Membrane proteins allow cell communication, act as transport proteins, enzymes, receptors, and provide cell identity markers.
What are the differences between integral and peripheral proteins?
Integral proteins span the membrane with hydrophobic and hydrophilic regions; peripheral proteins are loosely attached and involved in support, enzymes, motor functions, and cell recognition.
Name and describe the three types of cell junctions.
Tight junctions prevent fluid passage; desmosomes provide mechanical strength; gap junctions allow small molecules and electrical signals to pass between cells.
What is passive transport and its types?
Passive transport requires no energy and moves substances down their concentration gradient. Types include simple diffusion, facilitated diffusion, osmosis, and filtration.
Explain simple diffusion.
Simple diffusion is the movement of hydrophobic substances like oxygen and carbon dioxide directly through the phospholipid bilayer down their concentration gradient.
What is facilitated diffusion and its two mechanisms?
Facilitated diffusion transports hydrophilic molecules via carrier-mediated proteins that change shape and channel-mediated proteins that form aqueous pores.
Define osmosis.
Osmosis is the movement of water across a selectively permeable membrane from areas of low solute concentration to high solute concentration.
What is tonicity and its types?
Tonicity is a solution's ability to change cell shape by altering water volume: isotonic (no change), hypertonic (cell shrinks), and hypotonic (cell swells).
What distinguishes active transport from passive transport?
Active transport moves solutes against their concentration gradient using ATP, while passive transport moves solutes down the gradient without energy.
Describe primary active transport.
Primary active transport uses ATP to change protein shape and pump solutes like ions across the membrane, e.g., Na+-K+ pump.
What is secondary active transport?
Secondary active transport uses energy stored in ion gradients created by primary transport to move other solutes indirectly.
Explain vesicular transport.
Vesicular transport moves large substances via vesicles: endocytosis brings substances in, exocytosis expels substances out of the cell.
What is resting membrane potential (RMP)?
RMP is the electrical potential energy from charge separation across the plasma membrane, typically around -70 to -90 mV inside the cell.
Which ion is key in establishing RMP and why?
Potassium (K+) is key because it leaks out of the cell, leaving behind negatively charged proteins, making the inside more negative.
Name the membranous organelles in a cell.
Membranous organelles include the nucleus, mitochondria, endoplasmic reticulum, Golgi apparatus, peroxisomes, and lysosomes.
What is the function of the mitochondria?
Mitochondria produce most of the cell's ATP through aerobic cellular respiration and contain their own DNA and ribosomes.
Describe the rough and smooth endoplasmic reticulum (ER).
Rough ER has ribosomes and synthesizes proteins; smooth ER lacks ribosomes and functions in lipid synthesis and detoxification.
What is the role of the Golgi apparatus?
The Golgi apparatus modifies, concentrates, and packages proteins and lipids received from the rough ER for transport.
What are ribosomes and their types?
Ribosomes are protein synthesis sites made of rRNA and protein; they can be free in cytoplasm or membrane-bound on rough ER.
What are cilia, flagella, and microvilli?
Cilia are hair-like extensions that move substances across cell surfaces; flagella propel the whole cell; microvilli increase surface area for absorption.
What are the stages of mitosis?
Mitosis stages are prophase, metaphase, anaphase, and telophase, ensuring DNA is equally divided into daughter cells.
What is the central dogma of molecular biology in protein synthesis?
DNA is transcribed into RNA, which is then translated into proteins, following the sequence: DNA → RNA → Protein.
What is transcription?
Transcription is the process of copying DNA's base sequence into complementary mRNA in the nucleus.
What is translation?
Translation is decoding mRNA into a polypeptide chain at the ribosome using tRNA and the genetic code.
What is the role of tRNA in translation?
tRNA carries specific amino acids and uses its anticodon to pair with mRNA codons, adding amino acids to the growing protein.