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General Biology: Cell Structure and Membrane Function

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  • Compare prokaryotic and eukaryotic cells

    Prokaryotic cells lack membrane-bound organelles and a nucleus; eukaryotic cells have membrane-bound organelles and a nucleus. Both have plasma membranes and ribosomes.

  • What is the plasma membrane and which cells have it?

    The plasma membrane is a phospholipid bilayer that controls entry and exit of substances. Both prokaryotic and eukaryotic cells have a plasma membrane.

  • Structure and function of the nucleus

    The nucleus contains DNA, controls cell activities, and is surrounded by a double membrane with pores for transport.

  • Function of ribosomes in eukaryotic cells

    Ribosomes synthesize proteins by translating mRNA; they can be free in cytoplasm or bound to the rough ER.

  • Components of the endomembrane system

    Includes vesicles, ER (smooth and rough), Golgi apparatus, lysosomes, and vacuoles, which work together to modify, package, and transport proteins and lipids.

  • Difference between smooth and rough ER

    Rough ER has ribosomes and synthesizes proteins; smooth ER lacks ribosomes and synthesizes lipids and detoxifies chemicals.

  • Function of the Golgi apparatus

    The Golgi apparatus modifies, sorts, and packages proteins and lipids for secretion or delivery to other organelles.

  • Role of lysosomes

    Lysosomes contain digestive enzymes to break down macromolecules and damaged organelles.

  • Function of vacuoles in plant cells

    Vacuoles store nutrients, waste products, and help maintain turgor pressure in plant cells.

  • Organelles involved in energy transformation

    Mitochondria generate ATP via cellular respiration; chloroplasts conduct photosynthesis; peroxisomes detoxify harmful substances.

  • Structure and function of the cytoskeleton

    The cytoskeleton provides cell shape, support, and movement; includes microtubules, microfilaments, and intermediate filaments.

  • Role of motor proteins

    Motor proteins move organelles and vesicles along cytoskeletal tracks using ATP.

  • Extracellular components and connections

    Includes cell wall, extracellular matrix, and cell junctions like plasmodesmata, tight junctions, desmosomes, and gap junctions for communication and support.

  • Phospholipid structure and bilayer formation

    Phospholipids have hydrophilic heads and hydrophobic tails, forming a bilayer that creates a semi-permeable membrane.

  • Fluid mosaic model of the cell membrane

    The membrane is a fluid bilayer with embedded proteins, allowing lateral movement and dynamic structure.

  • Effect of saturated vs. unsaturated fatty acid tails on membrane fluidity

    Saturated tails make membranes less fluid and more viscous; unsaturated tails increase fluidity by preventing tight packing.

  • Role of cholesterol in membrane fluidity

    Cholesterol stabilizes membrane fluidity by preventing tight packing in cold and restraining movement in heat.

  • Types and functions of membrane proteins

    Integral proteins span the membrane; peripheral proteins attach to surfaces; functions include transport, enzymatic activity, and cell signaling.

  • Selective permeability of the cell membrane

    Small nonpolar molecules pass easily; large or polar molecules require transport proteins; ions need channels or carriers.

  • Passive transport mechanisms

    Includes diffusion, osmosis, and facilitated diffusion; moves substances down concentration gradients without energy.

  • Differences between isotonic, hypertonic, and hypotonic solutions

    Isotonic: equal solute concentration; hypertonic: higher solute outside, causing cell shrinkage; hypotonic: lower solute outside, causing cell swelling.

  • Water balance in plant vs. animal cells in different tonicities

    Plant cells become turgid in hypotonic, plasmolyzed in hypertonic; animal cells lyse in hypotonic and shrivel in hypertonic solutions.

  • Water potential and its role in water movement

    Water potential predicts water movement; water moves from higher to lower water potential; calculated as \(\Psi = \Psi_s + \Psi_p\).

  • Facilitated diffusion and protein roles

    Uses channel proteins for specific passage and carrier proteins for conformational transport; no energy required.

  • Why active transport requires energy

    Active transport moves substances against their concentration gradient, requiring ATP to power transport proteins.

  • Function of proton pumps

    Proton pumps use ATP to move H+ ions across membranes, creating electrochemical gradients.

  • Cotransport mechanism

    Cotransport uses the gradient of one molecule to drive the transport of another molecule against its gradient.

  • Bulk transport: exocytosis and endocytosis

    Exocytosis exports large molecules via vesicles; endocytosis imports substances by vesicle formation.