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General Biology: DNA Structure, Function, and Protein Synthesis

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  • What is the role of mRNA in protein synthesis?

    mRNA carries the genetic code from DNA in the nucleus to the ribosome, where it directs the sequence of amino acids in a protein.

  • What function does tRNA serve during translation?

    tRNA transfers specific amino acids to the ribosome and matches its anticodon with codons on the mRNA to ensure correct protein assembly.

  • What is the role of ribosomal RNA (rRNA)?

    rRNA is a structural and catalytic component of ribosomes, facilitating the assembly of amino acids into protein chains.

  • Define a codon.

    A codon is a sequence of three nucleotides on mRNA that specifies a particular amino acid or a stop signal during translation.

  • What is an anticodon?

    An anticodon is a set of three nucleotides on tRNA complementary to an mRNA codon, ensuring the correct amino acid is added to the growing protein.

  • What is the start codon and its significance?

    The start codon (usually AUG) signals the beginning of translation and codes for the amino acid methionine.

  • What are stop codons?

    Stop codons (UAA, UAG, UGA) signal the termination of translation, releasing the completed polypeptide chain.

  • Describe the main steps of translation.

    Translation involves initiation (ribosome assembly at start codon), elongation (amino acid chain growth), and termination (release at stop codon).

  • How does transcription differ from translation?

    Transcription copies DNA into mRNA in the nucleus; translation uses mRNA to synthesize proteins at the ribosome.

  • What is DNA replication?

    DNA replication is the process of copying the DNA molecule to produce two identical DNA strands before cell division.

  • What is the chemical composition difference between DNA and RNA?

    DNA contains deoxyribose sugar and thymine; RNA contains ribose sugar and uracil instead of thymine.

  • What is the sugar-phosphate backbone?

    The sugar-phosphate backbone forms the structural framework of DNA and RNA strands, linking nucleotides together.

  • What are exons and introns?

    Exons are coding sequences in RNA that remain after splicing; introns are non-coding sequences removed during RNA processing.

  • What is RNA splicing?

    RNA splicing removes introns from pre-mRNA, joining exons to form mature mRNA ready for translation.

  • What is a mutation?

    A mutation is a change in the DNA sequence that can affect protein function and organism traits.

  • Compare insertion, deletion, and substitution mutations.

    Insertion adds nucleotides, deletion removes nucleotides, and substitution replaces one nucleotide with another.

  • What are mutagens?

    Mutagens are physical or chemical agents that increase the rate of mutations in DNA.

  • How can mutations be harmful or beneficial?

    Mutations can disrupt protein function causing disease or provide genetic variation that may improve survival.

  • What is the genetic code?

    The genetic code is the set of rules by which nucleotide triplets (codons) specify amino acids in proteins.

  • What is the role of RNA polymerase in transcription?

    RNA polymerase synthesizes RNA by reading the DNA template strand during transcription.

  • What is a promoter in gene expression?

    A promoter is a DNA sequence where RNA polymerase binds to initiate transcription.

  • What is a terminator in transcription?

    A terminator is a DNA sequence signaling RNA polymerase to stop transcription.