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General Biology: Energy, Cell Cycle, Genetics, and Molecular Biology

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  • Define energy

    Energy is the capacity to do work or cause change.

  • Distinguish potential and kinetic energy

    Potential energy is stored energy; kinetic energy is energy of motion.

  • What is ATP's role in the cell?

    ATP is the only form of chemical fuel the cell can use directly for energy.

  • Define entropy

    Entropy is a measure of disorder or randomness in a system.

  • What powers most life on Earth?

    Most life on Earth is powered by energy from the sun.

  • Distinguish producers vs consumers

    Producers make their own food (e.g., plants); consumers obtain energy by eating other organisms.

  • Basics of photosynthesis

    Photosynthesis converts light energy into chemical energy stored in glucose.

  • Distinguish light reactions and Calvin cycle and their locations

    Light reactions occur in the thylakoid membranes and produce ATP and NADPH; Calvin cycle occurs in the stroma and synthesizes glucose.

  • Basics of cellular respiration

    Cellular respiration breaks down glucose to produce ATP.

  • Distinguish glycolysis, Krebs cycle, and electron transport chain and their locations

    Glycolysis occurs in the cytoplasm; Krebs cycle in the mitochondrial matrix; Electron transport chain in the inner mitochondrial membrane.

  • How are photosynthesis and cellular respiration linked?

    Photosynthesis produces glucose and oxygen used in cellular respiration; cellular respiration produces CO2 and water used in photosynthesis.

  • Difference between aerobic and anaerobic metabolism

    Aerobic metabolism requires oxygen; anaerobic metabolism does not.

  • Define calorie in nutrition and energy usage

    A calorie is the amount of energy needed to raise 1 gram of water by 1°C; used to measure food energy.

  • Where do carbohydrates, amino acids, and fats enter cellular respiration?

    Carbohydrates enter as glucose; amino acids enter at various points after deamination; fats enter as acetyl-CoA after beta-oxidation.

  • What is fermentation in anaerobic metabolism?

    Fermentation is a process that allows ATP production without oxygen by regenerating NAD+.

  • By-products of anaerobic glucose metabolism in animals

    Animals produce lactic acid as a by-product of anaerobic glucose metabolism.

  • By-products of fat and amino acid metabolism for energy in animals

    Fat metabolism produces acetyl-CoA; amino acid metabolism produces various intermediates entering cellular respiration.

  • Describe cell theory

    All living things are made of cells; cells are the basic unit of life; all cells come from pre-existing cells.

  • Define gametes and gene

    Gametes are reproductive cells; a gene is a DNA segment coding for a trait.

  • Distinguish sexual and asexual reproduction

    Sexual reproduction involves two parents and genetic variation; asexual reproduction involves one parent and produces clones.

  • DNA structure and function

    DNA is a double helix of nucleotides storing genetic information.

  • Chromatin vs chromosome

    Chromatin is loosely packed DNA in the nucleus; chromosomes are tightly packed DNA visible during cell division.

  • Phases of the cell cycle

    The cell cycle includes interphase (growth and DNA replication) and mitosis (cell division).

  • Major events in mitosis phases

    Prophase: chromosomes condense; Metaphase: chromosomes align; Anaphase: sister chromatids separate; Telophase: nuclei reform; Cytokinesis: cytoplasm divides.

  • Compare mitosis in animal vs plant cells

    Animal cells form a cleavage furrow; plant cells form a cell plate during cytokinesis.

  • Meiosis events and differences from mitosis

    Meiosis has two divisions producing four haploid cells; mitosis produces two diploid cells.

  • Three sources of genetic variation from sexual reproduction

    Crossing over, independent assortment, and random fertilization.

  • Genetic variation and survival

    Genetic variation increases survival by enabling adaptation to changing environments.

  • Basic Mendelian genetics

    Traits are inherited as dominant or recessive alleles following predictable ratios.

  • Complete vs incomplete dominance

    Complete dominance: one allele masks the other; incomplete dominance: heterozygote shows intermediate phenotype.

  • Sex-linked traits

    Traits associated with genes on sex chromosomes, often X-linked and more common in males.

  • Nucleotide structure and DNA formation

    Nucleotides consist of a sugar, phosphate, and base; they form DNA strands by phosphodiester bonds.

  • DNA to mRNA to amino acid order

    Transcription copies DNA to mRNA; translation uses mRNA to assemble amino acids into proteins.

  • Transcription vs translation

    Transcription occurs in the nucleus making mRNA; translation occurs in the cytoplasm making proteins.

  • Why DNA changes don't always change proteins

    Some mutations are silent due to redundancy in the genetic code or occur in non-coding regions.

  • Basic gene regulation

    Genes can be turned on or off by regulatory proteins controlling transcription.

  • How mutation can lead to cancer growth

    Mutations in genes controlling cell division can cause uncontrolled cell growth (cancer).

  • Genetic engineering vs genetic modification in agriculture

    Genetic engineering involves direct DNA manipulation; genetic modification includes traditional breeding and engineering.