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General Biology: Energy, Enzymes, Cellular Respiration, and Photosynthesis

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  • What is kinetic energy?

    Kinetic energy is the energy associated with motion. Moving objects perform work by imparting motion to matter.

  • How efficient are energy transformations in cellular metabolism?

    Only about 30–40% of the energy from a fuel molecule like glucose is captured in ATP or usable forms; the rest is lost as heat.

  • What is the structure and function of ATP?

    ATP consists of adenine, ribose sugar, and three phosphate groups. It is the key molecule for energy storage and transfer in cells.

  • What is a catabolic reaction?

    A catabolic reaction releases energy by breaking down complex molecules into simpler ones, such as in cellular respiration.

  • What is an anabolic reaction?

    An anabolic reaction requires energy to build large molecules from smaller ones, like in photosynthesis.

  • How do enzymes speed up reactions?

    Enzymes catalyze reactions by lowering the activation energy barrier, allowing reactants to reach the transition state at moderate temperatures.

  • Difference between competitive and noncompetitive inhibition?

    Competitive inhibitors block the active site, preventing substrate binding. Noncompetitive inhibitors bind elsewhere, changing enzyme shape and reducing activity.

  • What is an endergonic reaction?

    An endergonic reaction absorbs free energy from the surroundings and is nonspontaneous, requiring energy input.

  • What is an exergonic reaction?

    An exergonic reaction releases free energy to the surroundings and is spontaneous.

  • What is feedback inhibition?

    Feedback inhibition occurs when the end product of a metabolic pathway inhibits an earlier step to prevent overproduction, e.g., cholesterol synthesis regulation.

  • What is the overall reaction for cellular respiration?

    Cellular respiration: C6H12O6 + 6O2 → 6CO2 + 6H2O + ATP. It is a catabolic reaction occurring in mitochondria.

  • Do plants undergo cellular respiration?

    Yes, plants perform cellular respiration. Without oxygen or sunlight, they ferment, producing ethanol.

  • What are redox reactions in cellular respiration?

    Redox reactions transfer electrons between molecules; oxidation is loss of electrons, reduction is gain.

  • What happens during glycolysis and how many ATP are produced?

    Glycolysis splits glucose into 2 pyruvate in the cytoplasm, producing a net of 2 ATP and 2 NADH.

  • What occurs in the Krebs cycle and ATP yield?

    The Krebs cycle oxidizes acetyl-CoA to CO2, producing 1 ATP, NADH, and FADH2 per turn; it runs twice per glucose.

  • What happens in the electron transport chain (ETC)?

    ETC passes electrons from NADH and FADH2 through protein complexes, pumping H+ to create a gradient used by ATP synthase to make ~26-28 ATP; oxygen is final electron acceptor.

  • Role of NAD and FAD in cellular respiration?

    NAD and FAD transport electrons to the electron transport chain.

  • What is oxygen's role in cellular respiration?

    Oxygen acts as the final electron acceptor in the ETC, forming water.

  • What occurs during lactic acid fermentation?

    In absence of oxygen, pyruvate is converted to lactic acid, regenerating NAD+ for glycolysis and producing 2 ATP.

  • What occurs during alcoholic fermentation?

    Without oxygen, pyruvate converts to acetaldehyde and then ethanol, releasing CO2 and regenerating NAD+ for glycolysis.

  • Summarize the three steps of cellular respiration.

    Glycolysis (cytoplasm): glucose → 2 pyruvate + 2 ATP + 2 NADH. Krebs cycle (mitochondrial matrix): acetyl-CoA → CO2 + 2 ATP + NADH + FADH2. ETC (inner membrane): NADH/FADH2 donate electrons, H+ gradient drives ATP synthase, oxygen forms water.

  • What is the photosynthesis reaction and where does it occur?

    6CO2 + 6H2O + light → C6H12O6 + 6O2. It is anabolic and occurs in chloroplasts.

  • How do gases enter and exit leaves?

    Gases move through stomata, openings controlled by guard cells that regulate CO2 intake, O2 release, and water vapor loss.

  • Where are photosynthetic pigments located?

    Photosynthetic pigments like chlorophyll are in the thylakoid membranes of chloroplasts, organized in photosystems.

  • What happens during light-dependent reactions?

    Light excites electrons, water is split, producing ATP, NADPH, and releasing oxygen.

  • What happens during light-independent reactions (Calvin cycle)?

    Occurs in chloroplast stroma; uses ATP and NADPH to fix CO2 into glucose without direct light.

  • How do electrons flow during photosynthesis?

    Electrons move through the thylakoid membrane photosystems, powering ATP and NADPH production for the Calvin cycle.

  • What products from light-dependent reactions feed the Calvin cycle?

    The Calvin cycle receives ATP and NADPH produced in light-dependent reactions.

  • Which light colors drive photosynthesis and why is the leaf green?

    Photosynthesis mainly uses red and blue light because chlorophyll absorbs these; leaves appear green because chlorophyll reflects green light.