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General Biology Key Concepts

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  • Range of object sizes for light microscope

    Light microscopes visualize objects roughly from 200 nm to 1 mm; smaller structures require electron microscopes.
  • Difference between SEM and TEM

    SEM scans surfaces producing 3D images; TEM transmits electrons through thin samples for internal structure.
  • Purpose of stains in light microscopy

    Stains increase contrast by coloring specific cell components, making structures visible under light microscopes.
  • Function of cell fractionation

    Cell fractionation separates cellular components by size and density for detailed study.
  • Organelles found in both plant and animal cells

    Nucleus, mitochondria, ER, Golgi apparatus, ribosomes, peroxisomes, cytoskeleton.
  • Organelles unique to plant cells

    Chloroplasts, central vacuole, cell wall, plasmodesmata.
  • Role of ribosomes

    Ribosomes synthesize proteins by translating mRNA.
  • Nuclear envelope and nuclear pores

    The nuclear envelope is a double membrane surrounding the nucleus; nuclear pores regulate molecule passage.
  • Functions of smooth and rough ER

    Smooth ER synthesizes lipids and detoxifies; rough ER has ribosomes and synthesizes proteins.
  • Role of the Golgi apparatus

    Modifies, sorts, and packages proteins and lipids for secretion or delivery.
  • What are lysosomes?

    Lysosomes contain enzymes that digest macromolecules and recycle cellular waste.
  • Phagocytosis definition

    Phagocytosis is the process of engulfing large particles or cells for digestion.
  • What is the tonoplast?

    The tonoplast is the membrane surrounding the central vacuole in plant cells.
  • Function of mitochondria

    Mitochondria generate ATP through cellular respiration.
  • Endosymbiont theory evidence

    Mitochondria and chloroplasts have double membranes, own DNA, and reproduce independently.
  • Components of the cytoskeleton

    Microtubules (tubulin), microfilaments (actin), intermediate filaments (varied proteins).
  • Function of motor proteins kinesin and dynein

    They move cargo along microtubules using ATP.
  • Structural organization of cilia and flagella

    9+2 arrangement of microtubules with dynein arms for movement.
  • Primary vs secondary plant cell walls

    Primary walls are flexible and formed first; secondary walls are thicker and rigid.
  • Types of animal cell junctions

    Tight junctions (seal cells), desmosomes (anchor cells), gap junctions (communication).
  • Main components of the plasma membrane

    Phospholipid bilayer with embedded proteins, cholesterol, glycolipids, and glycoproteins.
  • Fluid mosaic model

    Membrane is a fluid bilayer of lipids with proteins floating within.
  • Difference between active and passive transport

    Active transport requires energy to move substances against gradients; passive transport does not.
  • Osmosis definition

    Diffusion of water across a selectively permeable membrane.
  • Membrane potential

    Voltage difference across a membrane due to ion distribution.
  • Sodium-potassium pump function

    Uses ATP to pump 3 Na+ out and 2 K+ into animal cells, maintaining gradients.
  • Catabolic vs anabolic pathways

    Catabolic pathways break down molecules releasing energy; anabolic build molecules consuming energy.
  • First and second laws of thermodynamics

    1st: Energy conserved; 2nd: Entropy increases in isolated systems.
  • Free energy and spontaneity

    Negative free energy change means spontaneous (exergonic); positive means nonspontaneous (endergonic).
  • Role of enzymes

    Enzymes lower activation energy to speed up reactions without being consumed.