Skip to main content
뒤로

General Biology: Key Concepts and Core Practicals

컨트롤 버튼이 '내비게이션' 모드로 변경되었습니다.
1/30
  • Difference between eukaryotic and prokaryotic cells

    Eukaryotic cells have a nucleus and membrane-bound organelles; prokaryotic cells lack a nucleus and have circular DNA and plasmids.

  • Function of the nucleus in eukaryotic cells

    Contains DNA coding for proteins needed to build new cells, enclosed in a nuclear membrane.

  • Role of mitochondria

    Site of aerobic respiration reactions, providing energy for the cell.

  • Function of ribosomes

    Where protein synthesis occurs, found on rough endoplasmic reticulum.

  • Special features of plant cells not in animal cells

    Chloroplasts for photosynthesis, permanent vacuole for rigidity, and cell wall made of cellulose.

  • Key structures in bacterial cells

    Cell wall made of peptidoglycan, cell membrane, cytoplasm, circular chromosomal DNA, plasmids, and sometimes flagella.

  • Definition of cell differentiation

    Process where cells gain new sub-cellular structures to become specialised for a specific function.

  • Adaptations of sperm cells

    Streamlined head, long tail for swimming, many mitochondria for energy, acrosome with enzymes, and haploid nucleus.

  • Function of root hair cells

    Specialised for water uptake by osmosis and mineral ions by active transport, with large surface area and mitochondria.

  • Purpose of xylem cells

    Transport water and minerals from roots to shoots; lignin deposits cause cells to die and form hollow tubes.

  • How phloem cells transport substances

    Carry products of photosynthesis through sieve plates; companion cells provide energy via mitochondria.

  • Maximum magnification and resolution of light microscopes

    Up to 2000x magnification and 200nm resolving power, used to view cells and large organelles.

  • Advantages of electron microscopes

    Use electrons for higher resolution (down to 0.2nm), magnification up to 2,000,000x, can view viruses and small organelles.

  • Formula for magnification

    \(\text{Magnification} = \frac{\text{size of image}}{\text{size of object}}\)

  • Lock and Key Hypothesis of enzyme action

    Substrate fits exactly into enzyme's active site forming enzyme-substrate complex, enabling the reaction.

  • Effect of temperature on enzyme activity

    Activity increases to an optimum (~37°C in humans), then decreases rapidly as enzymes denature above this.

  • Effect of pH on enzyme activity

    Enzymes have an optimum pH; too high or low pH changes active site shape, causing denaturation and loss of function.

  • How substrate concentration affects enzyme activity

    Rate increases with substrate concentration until saturation point, where all active sites are occupied.

  • Purpose of iodine in amylase activity practical

    Iodine turns blue-black in presence of starch, indicating starch breakdown by amylase over time.

  • Equation for rate of enzyme reaction

    \(\text{Rate} = \frac{\text{Change}}{\text{Time}}\)

  • Function of carbohydrases, proteases, and lipases

    Carbohydrases break carbs into sugars, proteases break proteins into amino acids, lipases break lipids into fatty acids and glycerol.

  • Food test for starch

    Add iodine solution; blue-black color indicates presence of starch.

  • Food test for reducing sugars

    Add Benedict's solution and heat; reddish-brown precipitate indicates reducing sugars.

  • Food test for protein

    Biuret test: add potassium hydroxide and copper sulfate; violet color indicates protein.

  • Food test for lipids

    Emulsion test: add ethanol and water; white emulsion layer indicates lipids.

  • Calorimetry equation for energy transferred

    \(\text{Energy} = \text{mass of water} \times 4.2 \times \text{temperature increase}\)

  • Definition of diffusion

    Passive movement of molecules from high to low concentration without energy.

  • Definition of osmosis

    Passive movement of water from dilute to concentrated solution across a selectively permeable membrane.

  • Definition of active transport

    Energy-requiring movement of molecules against concentration gradient using ATP.

  • Purpose of potato osmosis practical

    Measure percentage gain or loss of mass in potato disks placed in sucrose solutions of different concentrations.