Understand the cell cycle phases: The cell cycle consists of interphase (G1, S, G2 phases) and the mitotic phase (M phase), which includes prophase, metaphase, anaphase, and telophase.
Identify the role of sister chromatids: Sister chromatids are identical copies of a single chromosome that are connected by a centromere. They are crucial for accurate genetic material distribution during cell division.
Review the events of mitosis: During mitosis, the sister chromatids must be separated to ensure each daughter cell receives an identical set of chromosomes.
Focus on anaphase: In anaphase, the centromeres split, and the spindle fibers pull the sister chromatids apart toward opposite poles of the cell. This separation is a key event in mitosis.
Conclude that sister chromatids separate during anaphase, which is the phase following metaphase where the chromatids align at the cell's equatorial plane.