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Anatomy & Physiology: Cell Structure and Membrane Transport

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  • What are the three basic parts of a human cell?

    Plasma membrane: flexible outer boundary
    Cytoplasm: intracellular fluid containing organelles
    Nucleus: DNA-containing control center

  • Describe the structure of the plasma membrane.

    A double layer of phospholipids with embedded proteins, cholesterol, and carbohydrates forming a fluid mosaic model that controls what enters and leaves the cell.

  • What are the properties of phospholipid heads and tails in the membrane?

    Phosphate heads: polar, hydrophilic (water-loving)
    Fatty acid tails: nonpolar, hydrophobic (water-hating)

  • What role does cholesterol play in the plasma membrane?

    Located between phospholipid tails, cholesterol increases membrane stiffness and stability.

  • Differentiate between integral and peripheral membrane proteins.

    Integral proteins span the membrane and function as transporters, enzymes, or receptors.
    Peripheral proteins are loosely attached and function in enzymes, motor proteins, and cell connections.

  • What are the two main types of membrane transport?

    Passive transport: no energy required
    Active transport: energy (ATP) required to move substances against their concentration gradient.

  • What is simple diffusion?

    Movement of nonpolar, lipid-soluble substances directly through the lipid bilayer from high to low concentration without energy.

  • How does facilitated diffusion differ from simple diffusion?

    Facilitated diffusion uses carrier or channel proteins to transport specific hydrophobic molecules down their concentration gradient without energy.

  • What is the difference between carrier-mediated and channel-mediated facilitated diffusion?

    Carrier-mediated: protein changes shape to move large polar molecules.
    Channel-mediated: molecules move through aqueous channels based on size and charge.

  • What is osmosis and how does it occur?

    Osmosis is the diffusion of water through a selectively permeable membrane via lipid bilayer or aquaporins, moving from low to high solute concentration.

  • Define tonicity and its effects on red blood cells.

    Tonicity is a solution's ability to change cell shape by altering water volume.
    Isotonic: normal shape
    Hypertonic: cell shrinks (crenation)
    Hypotonic: cell swells and may burst (lysis)

  • What is primary active transport and give an example?

    Transport using ATP to move solutes against their gradient.
    Example: Na+/K+ pump moves 3 Na+ out and 2 K+ in, maintaining electrochemical gradients.

  • Explain secondary active transport.

    Uses energy stored in ion gradients created by primary active transport to move other solutes indirectly, e.g., Na+ gradient driving glucose uptake.

  • What is vesicular transport and its types?

    Movement of large substances in vesicles requiring ATP.
    Types: Endocytosis, Exocytosis, Transcytosis, and Vesicular trafficking.

  • Describe endocytosis and its selectivity.

    Process of engulfing substances into the cell via protein-coated vesicles; can be selective if receptors bind specific molecules.

  • What is phagocytosis?

    "Cell eating" where pseudopods engulf solid particles forming a phagosome; used by macrophages and some white blood cells.

  • What is pinocytosis?

    "Cell drinking" where plasma membrane infolds to bring extracellular fluid and solutes inside; nonspecific and receptor-independent.

  • Explain receptor-mediated endocytosis.

    Selective uptake of molecules using plasma membrane receptors that bind specific substances like hormones or cholesterol.

  • What triggers exocytosis and how does it occur?

    Triggered by cell signals or membrane voltage changes; secretory vesicles fuse with membrane via v-SNARE and t-SNARE proteins to release contents.

  • Why is the plasma membrane selectively permeable?

    Its hydrophobic core allows nutrients in, keeps valuable substances inside, and lets wastes exit, controlling cellular environment.