객관식
Estimate the isoelectric point of the following dipeptide in the figure.
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4. Protein Structure
Isoelectric Point of a Peptide
객관식
Calculate the approximate pI of the peptide: C-G-E-K.
A
3.08
B
5.71
C
6.05
D
9.63
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검증된 단계별 안내1
Identify the ionizable groups in the peptide C-G-E-K. These include the N-terminus, the C-terminus, and the side chains of the amino acids Cysteine (C), Glutamic acid (E), and Lysine (K).
Determine the pKa values for each ionizable group. Typically, the pKa values are approximately: N-terminus (~9.0), C-terminus (~2.0), Cysteine (~8.3), Glutamic acid (~4.1), and Lysine (~10.5).
List the pKa values in ascending order: 2.0 (C-terminus), 4.1 (Glutamic acid), 8.3 (Cysteine), 9.0 (N-terminus), 10.5 (Lysine).
To find the isoelectric point (pI), identify the pKa values between which the peptide has a net charge of zero. This typically involves averaging the pKa values around the neutral charge state.
Calculate the pI by averaging the two pKa values that correspond to the transition from a positive to a neutral charge and from a neutral to a negative charge. In this case, average the pKa values of the N-terminus and Cysteine.
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