Which of the following functional groups of an amino acid would be in the ionized (deprotonated) state at high pH?
A
Amino group (\(-NH_2\))
B
Side chain of glycine
C
Hydrogen atom attached to the alpha carbon
D
Carboxyl group (\(-COO^-\))
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1
Understand the concept of pH and its effect on ionization: At high pH (basic conditions), the environment has a low concentration of hydrogen ions (\(\text{H}\)^+), which promotes deprotonation of acidic functional groups.
Identify the functional groups in an amino acid: Amino acids typically have an amino group (\(\text{-NH}\)_2), a carboxyl group (\(\text{-COOH}\)), a hydrogen atom attached to the alpha carbon, and a side chain (R group).
Determine the behavior of the carboxyl group (\(\text{-COOH}\)) at high pH: The carboxyl group is acidic and tends to lose a proton (\(\text{H}\)^+) under basic conditions, becoming ionized as \(\text{-COO}\)^-.
Analyze the amino group (\(\text{-NH}\)_2): The amino group is basic and tends to accept a proton (\(\text{H}\)^+) under acidic conditions, but at high pH, it remains in its neutral state (\(\text{-NH}\)_2) and does not ionize further.
Conclude that the carboxyl group (\(\text{-COO}\)^-) is the functional group that would be in the ionized (deprotonated) state at high pH, as it loses a proton and becomes negatively charged.