객관식
Salting out consists of adding __________ in order to ________________________.
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5. Protein Techniques
Salting Out
객관식
Which statement best explains the basis of salting out?
A
Presence of some salt ions weakens ionic interactions between proteins, leading to greater protein solubility.
B
Too few salt ions can deprive proteins of H2O solvent, leading to protein precipitation.
C
Addition of salt ions strengthens ionic interactions between proteins, leading to greater protein solubility.
D
Too many salt ions can deprive proteins of H2O solvent, leading to protein precipitation.
검증된 단계별 안내1
Understand the concept of 'salting out': Salting out is a technique used to precipitate proteins by adding high concentrations of salt to a solution. This process exploits the solubility properties of proteins in the presence of salt.
Recognize the role of salt ions: In a solution, salt ions compete with proteins for water molecules. When the concentration of salt is high, salt ions effectively 'steal' water molecules away from proteins.
Explain the effect on protein solubility: As salt ions bind water molecules, there are fewer water molecules available to solubilize proteins. This reduction in available solvent leads to decreased protein solubility.
Describe the precipitation process: With insufficient water to maintain solubility, proteins aggregate and precipitate out of the solution. This is the basis of the 'salting out' effect.
Clarify the correct statement: The correct explanation is that too many salt ions can deprive proteins of H2O solvent, leading to protein precipitation, as proteins become less soluble and aggregate.
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