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General Biology I Chapter 12: DNA Technology and Genetic Engineering

컨트롤 버튼이 '내비게이션' 모드로 변경되었습니다.
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  • Plasmid

    A small, circular DNA molecule found in bacteria used as a vector to carry foreign DNA into host cells during gene cloning.

  • Restriction enzyme

    An enzyme that cuts DNA at specific sequences called restriction sites, producing restriction fragments used in DNA cloning.

  • DNA ligase

    An enzyme that joins DNA fragments by forming phosphodiester bonds, used to seal foreign DNA into plasmids.

  • Gene cloning

    The process of producing multiple copies of a specific gene by inserting it into a plasmid and replicating it in a host organism.

  • Polymerase chain reaction (PCR)

    A technique used to amplify a specific DNA sequence exponentially using primers, DNA polymerase, and thermal cycling.

  • Gel electrophoresis

    A method to separate DNA or proteins by size using an electric field to move molecules through a gel matrix.

  • CRISPR-Cas9 system

    A gene editing tool that uses a guide RNA and Cas9 enzyme to make precise cuts in DNA for targeted modifications.

  • Genetically modified organisms (GMOs)

    Organisms whose genomes have been altered by genetic engineering to express desired traits, often used in agriculture.

  • DNA technology

    Techniques used to manipulate DNA for research, medicine, agriculture, and forensic applications.

  • Human Genome Project (HGP)

    An international research effort to sequence and map all human genes, providing a foundation for genomics and medicine.

  • Bioinformatics

    The use of computer technology to store, analyze, and interpret biological data, especially DNA sequences.

  • Complementary DNA (cDNA)

    DNA synthesized from an mRNA template using reverse transcriptase, representing expressed genes without introns.

  • DNA profiling

    A forensic technique that uses patterns in DNA, such as short tandem repeats (STRs), to identify individuals.

  • Short tandem repeat (STR)

    Repeating sequences of 2-5 base pairs in DNA used in genetic fingerprinting and DNA profiling.

  • Nucleic acid probe

    A labeled single-stranded DNA or RNA molecule used to detect complementary sequences in DNA samples.

  • Recombinant DNA

    DNA molecules formed by combining DNA from different sources, often used in genetic engineering.

  • Vector

    A DNA molecule, such as a plasmid, used to carry foreign DNA into a host cell for cloning or expression.

  • Gene therapy

    The insertion of genes into an individual's cells to treat or prevent disease.

  • Proteomics

    The large-scale study of proteins, their structures, and functions.

  • Whole-genome shotgun method

    A sequencing approach that breaks the genome into small fragments, sequences them, and assembles the sequences using computers.

  • Primers

    Short DNA sequences that provide a starting point for DNA synthesis during PCR.

  • Reverse transcriptase

    An enzyme that synthesizes DNA from an RNA template, used to create cDNA.

  • Forensics

    The application of DNA technology to legal cases, including identification and crime scene analysis.

  • Transgenic organism

    An organism that contains genes from another species introduced by genetic engineering.

  • Vaccine

    A preparation that stimulates the immune system to recognize and fight pathogens, sometimes produced using recombinant DNA technology.

  • Biotechnology

    The use of living organisms or their products to develop useful products and technologies.

  • DNA cloning

    The process of making multiple identical copies of a DNA fragment.

  • Repetitive DNA

    Sequences that are repeated many times in the genome, often used in DNA profiling.