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Ch. 20 - DNA Tools and Biotechnology
Campbell - Campbell Biology 12th Edition
Urry12th EditionCampbell BiologyISBN: 9785794169850당신이 사용하는 게 아니라요?교과서 변경
모든 교과서Urry 12th EditionCh. 20 - DNA Tools and Biotechnology문제 2
20장, 문제 2

Which of the following tools of DNA technology is incorrectly paired with its use?
a. Electrophoresis — Separation of DNA fragments
b. DNA ligase — Cutting DNA, creating sticky ends of restriction fragments
c. DNA polymerase — Polymerase chain reaction to amplify sections of DNA
d. Reverse transcriptase — Production of cDNA from mRNA

검증된 단계별 안내
1
Understand the function of each tool mentioned in the options: Electrophoresis, DNA ligase, DNA polymerase, and reverse transcriptase. Each tool has a specific role in DNA technology.
Option a: Electrophoresis is used to separate DNA fragments based on their size by applying an electric field. Smaller fragments move faster through the gel. This pairing is correct.
Option b: DNA ligase is an enzyme that joins DNA fragments by forming phosphodiester bonds. It does not cut DNA or create sticky ends; that is the role of restriction enzymes. This pairing is incorrect.
Option c: DNA polymerase is used in the polymerase chain reaction (PCR) to amplify specific sections of DNA by synthesizing new DNA strands. This pairing is correct.
Option d: Reverse transcriptase is used to synthesize complementary DNA (cDNA) from an mRNA template. This is a key step in studying gene expression. This pairing is correct.

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주요 개념

질문에 올바르게 답하기 위해 반드시 이해해야 하는 핵심 개념들은 다음과 같습니다.

Electrophoresis

Electrophoresis is a laboratory technique used to separate DNA fragments based on their size and charge. In this process, an electric current is applied to a gel matrix, causing the negatively charged DNA to migrate towards the positive electrode. Smaller fragments move faster through the gel, allowing for the visualization and analysis of different DNA sizes.
추천 영상:
07:58
Gel Electrophoresis

DNA Ligase

DNA ligase is an enzyme that facilitates the joining of DNA strands together by forming phosphodiester bonds. It is crucial in processes such as DNA replication and repair, as well as in recombinant DNA technology, where it is used to connect DNA fragments. However, it does not cut DNA; that function is performed by restriction enzymes.
추천 영상:
02:09
Step 1) Create Recombinant DNA

Reverse Transcriptase

Reverse transcriptase is an enzyme that synthesizes complementary DNA (cDNA) from an RNA template. This process is essential in molecular biology for converting mRNA into cDNA, which can then be amplified and analyzed. It is commonly used in applications such as cloning and gene expression studies, particularly in the context of retroviruses.
추천 영상:
04:16
1) Initiation of Transcription
관련 실천
교과서 질문

In DNA technology, the term vector can refer to

a. The enzyme that cuts DNA into restriction fragments.

b. The sticky end of a DNA fragment.

c. A SNP marker.

d. A plasmid used to transfer DNA into a living cell.

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교과서 질문

Which of the following is true of cDNA produced using human brain tissue as the starting material?

a. The procedure to make it requires amplification by the polymerase chain reaction.

b. It is produced from pre-mRNA using reverse transcriptase.

c. It can be labeled and used as a probe to detect genes expressed in the brain.

d. It includes the introns of the pre-mRNA.

1166
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교과서 질문

Plants are more readily manipulated by genetic engineering than are animals because

a. Plant genes do not contain introns.

b. More vectors are available for transferring recombinant DNA into plant cells.

c. A somatic plant cell can often give rise to a complete plant.

d. Plant cells have larger nuclei.

2112
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교과서 질문

A paleontologist has recovered a bit of tissue from the 400-year-old preserved skin of an extinct dodo (a bird). To compare a specific region of the DNA from a sample with DNA from living birds, which of the following would be most useful for increasing the amount of dodo DNA available for testing?

a. SNP analysis

b. Polymerase chain reaction (PCR)

c. Electroporation

d. Gel electrophoresis

1712
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