Skip to main content
Ch. 8 - Molecular Biology of Transcription and RNA Processing
Sanders - Genetic Analysis: An Integrated Approach 3rd Edition
Sanders3rd EditionGenetic Analysis: An Integrated ApproachISBN: 9780135564172당신이 사용하는 게 아니라요?교과서 변경
8장, 문제 16b

The segment of the bacterial TrpA gene involved in intrinsic termination of transcription is the following:
3'-TGGGTCGGGGCGGATTACTGCCCCGAAAAAAAACTTG-5'
5'-ACCCAGCCCCGCCTAATGACGGGGCTTTTTTTTGAAC-3'
Label the template and coding DNA strands.

검증된 단계별 안내
1
Determine the orientation of the DNA strands: The template strand is the one that RNA polymerase reads to synthesize RNA, while the coding strand has the same sequence as the RNA (except thymine is replaced with uracil in RNA).
Identify the 5' to 3' direction for each strand: The sequence provided shows the 3'-5' direction for the first strand and the 5'-3' direction for the second strand.
Label the template strand: The template strand is the one that is complementary to the RNA being synthesized. In this case, the 3'-TGGGTCGGGGCGGATTACTGCCCCGAAAAAAAACTTG-5' strand is the template strand.
Label the coding strand: The coding strand is the one that matches the RNA sequence (except for uracil replacing thymine). In this case, the 5'-ACCCAGCCCCGCCTAATGACGGGGCTTTTTTTTGAAC-3' strand is the coding strand.
Verify the labeling: Ensure that the template strand is complementary to the coding strand and that the coding strand matches the RNA sequence (with uracil replacing thymine).

비슷한 문제에 대한 검증된 영상 답변:

이 영상 해법은 위 문제에 도움이 된다고 튜터들이 추천한 것입니다.
영상 길이:
3m

주요 개념

질문에 올바르게 답하기 위해 반드시 이해해야 하는 핵심 개념들은 다음과 같습니다.

DNA Strands

DNA consists of two strands: the template strand and the coding strand. The template strand is used by RNA polymerase to synthesize RNA during transcription, while the coding strand has the same sequence as the RNA (except for thymine being replaced by uracil). Understanding the roles of these strands is crucial for analyzing transcription processes.
추천 영상:
가이드 코스
03:03
Double Strand Breaks

Transcription

Transcription is the process by which RNA is synthesized from a DNA template. It involves the enzyme RNA polymerase binding to the DNA at a promoter region, unwinding the DNA, and synthesizing a complementary RNA strand. Recognizing the significance of transcription is essential for understanding gene expression and regulation.
추천 영상:
가이드 코스
09:16
Eukaryotic Transcription

Intrinsic Termination

Intrinsic termination is a mechanism that signals the end of transcription in prokaryotes. It occurs when a specific sequence in the RNA forms a hairpin structure followed by a series of uracils, causing RNA polymerase to dissociate from the DNA. This concept is vital for understanding how transcription is precisely regulated and terminated.
추천 영상:
가이드 코스
01:55
Translation Termination
관련 실천
교과서 질문

A 2-kb fragment of E. coli DNA contains the complete sequence of a gene for which transcription is terminated by the rho protein. The fragment contains the complete promoter sequence as well as the terminator region of the gene. The cloned fragment is examined by band shift assay. Each lane of a single electrophoresis gel contains the 2-kb cloned fragment under the following conditions:

Lane 1: 2-kb fragment alone

Lane 2: 2-kb fragment plus the core enzyme

Lane 3: 2-kb fragment plus the RNA polymerase holoenzyme

Lane 4: 2-kb fragment plus rho protein

Explain the relative positions of bands in lanes 1 and 3.

567
views
교과서 질문

The segment of the bacterial TrpA gene involved in intrinsic termination of transcription is the following:

3'-TGGGTCGGGGCGGATTACTGCCCCGAAAAAAAACTTG-5'

5'-ACCCAGCCCCGCCTAATGACGGGGCTTTTTTTTGAAC-3'

Explain how a sequence of this type leads to intrinsic termination of transcription.

555
views
교과서 질문

A 2-kb fragment of E. coli DNA contains the complete sequence of a gene for which transcription is terminated by the rho protein. The fragment contains the complete promoter sequence as well as the terminator region of the gene. The cloned fragment is examined by band shift assay. Each lane of a single electrophoresis gel contains the 2-kb cloned fragment under the following conditions:

Lane 1: 2-kb fragment alone

Lane 2: 2-kb fragment plus the core enzyme

Lane 3: 2-kb fragment plus the RNA polymerase holoenzyme

Lane 4: 2-kb fragment plus rho protein

Diagram the relative positions expected for the DNA fragments in this gel electrophoresis analysis.

592
views
교과서 질문

The eukaryotic gene Gen-100 contains four introns labeled A to D. Imagine that Gen-100 has been isolated and its DNA has been denatured and mixed with polyadenylated mRNA from the gene.

Are intron regions single stranded or double stranded? Why?

661
views
교과서 질문

The segment of the bacterial TrpA gene involved in intrinsic termination of transcription is the following:

3'-TGGGTCGGGGCGGATTACTGCCCCGAAAAAAAACTTG-5'

5'-ACCCAGCCCCGCCTAATGACGGGGCTTTTTTTTGAAC-3' Draw the mRNA structure that forms during transcription of this segment of the TrpA gene.

548
views
교과서 질문

The eukaryotic gene Gen-100 contains four introns labeled A to D. Imagine that Gen-100 has been isolated and its DNA has been denatured and mixed with polyadenylated mRNA from the gene.

Label the introns.

587
views