Which of the following techniques results in isolated colonies both within the agar and on top of the agar?
A
Serial dilution without plating
B
Pour plate technique
C
Streak plate technique
D
Spread plate technique
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Understand the goal: The question asks which microbiological technique results in isolated colonies both within the agar and on top of the agar surface.
Recall the characteristics of each technique: Serial dilution without plating does not involve growing colonies on agar, so it cannot produce isolated colonies on or within agar.
Consider the streak plate technique: This method spreads bacteria on the surface of the agar to obtain isolated colonies only on the surface, not within the agar.
Consider the spread plate technique: This involves spreading a diluted sample on the surface of the agar, resulting in isolated colonies only on the surface.
Consider the pour plate technique: This involves mixing the diluted sample with molten agar and then pouring it into a petri dish, which allows colonies to grow both within the agar and on its surface, leading to isolated colonies in both locations.