객관식
Cells that have the ability to take up DNA from the environment are called ______ cells.
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16. Microbial Genetics
Bacterial Transformation
객관식
DNA transformation in bacteria is an important tool for DNA modification and cloning experiments. If the DNA being transformed by the bacteria contains antibiotic resistance genes, how does this help the scientists?
A
Antibiotics will kill any bacteria that did not naturally transform the transferred DNA.
B
It allows the scientists to identify which bacterial cells are competent and which cells are not.
C
It allows scientists to remove all bacteria that have not incorporated the experimental genes into their genome.
D
All of the above are reasons why antibiotic resistance is commonly used in transformation experiments.
검증된 단계별 안내1
Understand the concept of DNA transformation: DNA transformation is a process where bacteria take up foreign DNA from their environment. This is a key technique in genetic engineering and molecular biology.
Recognize the role of antibiotic resistance genes: These genes are often included in the DNA being transformed because they provide a selectable marker. Only bacteria that have successfully taken up and expressed the foreign DNA will survive in the presence of the antibiotic.
Identify the purpose of using antibiotics: After transformation, the bacteria are grown on media containing the antibiotic. This step ensures that only the bacteria that have incorporated the DNA with the resistance gene will survive, effectively selecting for transformed cells.
Consider the implications for experimental design: By using antibiotic resistance, scientists can easily distinguish between bacteria that have been successfully transformed and those that have not, as only the former will grow in the presence of the antibiotic.
Conclude with the benefits of this method: The use of antibiotic resistance genes in transformation experiments allows for efficient selection and identification of transformed bacteria, making it a powerful tool in genetic research and biotechnology.
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