객관식
When performing a Gram stain on Bacillus megaterium, what staining result is expected under the microscope?
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- 1. Introduction to Microbiology3h 27m
- Introduction to Microbiology18m
- Introduction to Taxonomy26m
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- Gram Stain13m
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9. Microscopes
Gram Stain
Multiple Choice
In the Gram stain procedure, which dye is used as the counterstain?
A
Iodine (Gram's iodine)
B
Crystal violet
C
Acetone-alcohol decolorizer
D
Safranin
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검증된 단계별 안내1
Understand the purpose of each reagent in the Gram stain procedure: Crystal violet is the primary stain that colors all cells purple initially.
Recognize that Gram's iodine acts as a mordant, forming a complex with crystal violet to fix the dye inside the cell walls.
Know that acetone-alcohol is the decolorizer, which removes the primary stain from Gram-negative cells but not from Gram-positive cells.
Identify that the counterstain is applied after decolorization to stain the cells that lost the primary stain, providing contrast.
Recall that Safranin is the counterstain used in the Gram stain procedure to color Gram-negative bacteria pink or red.
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