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Which of the following immunoassay techniques would be the best for screening thousands of specimens for COVID-19?
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- 1. Introduction to Microbiology3h 27m
- Introduction to Microbiology18m
- Introduction to Taxonomy26m
- Scientific Naming of Organisms9m
- Members of the Bacterial World10m
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- Introduction to Archaea10m
- Introduction to Eukarya20m
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- The Human Microbiome46m
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- 1) Hide Within Host Cells5m
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- 26. Applications of the Immune Response2h 9m
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- Tests to Guide Antimicrobial Use21m
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- 29. Microbial Infections - Skin and Eyes46m
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- 33. Microbial Infections - Nervous System2h 18m
- 34. Microbial Infections - Urogenital System1h 40m
26. Applications of the Immune Response
Immunoassay: Western Blotting
Multiple Choice
All of the following immunoassay testing techniques are paired with their correct characteristics except which of these answers?
A
Fluorescent antibody test – microscopy.
B
ELISA – radioactive label.
C
Western blot – gel electrophoresis.
D
All of the above are matched correctly.
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검증된 단계별 안내1
Step 1: Understand the principle behind each immunoassay technique mentioned. The Fluorescent Antibody Test uses fluorescent dyes to label antibodies, which are then visualized under a fluorescence microscope, so it is correctly paired with microscopy.
Step 2: Review the ELISA (Enzyme-Linked Immunosorbent Assay) technique. ELISA uses an enzyme-linked antibody or antigen to produce a color change, not a radioactive label. Radioactive labels are characteristic of radioimmunoassays (RIA), so this pairing is incorrect.
Step 3: Examine the Western blot technique. Western blot involves separating proteins by gel electrophoresis, transferring them to a membrane, and detecting specific proteins with labeled antibodies, so it is correctly paired with gel electrophoresis.
Step 4: Compare all options to identify which pairing is incorrect. Since ELISA does not use radioactive labels, the statement 'ELISA – radioactive label' is the incorrect match.
Step 5: Conclude that the correct answer to the question is the ELISA paired with radioactive label, as it is the only mismatched pair among the options.
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