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Conjugation: F Plasmids quiz
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What is a conjugative plasmid?
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What is a conjugative plasmid?
A conjugative plasmid is a plasmid capable of directing its own transfer to a recipient cell via conjugation.
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하이드의 정의
What is a conjugative plasmid?
A conjugative plasmid is a plasmid capable of directing its own transfer to a recipient cell via conjugation.
What does the 'F' in F plasmid stand for?
The 'F' in F plasmid stands for fertility.
Which type of cell contains the entire F plasmid?
F+ cells contain the entire F plasmid.
What structure do F+ cells synthesize to initiate conjugation?
F+ cells synthesize an F pilus to initiate conjugation.
What is the role of F- cells in conjugation?
F- cells serve as recipient cells in conjugation because they lack the entire F plasmid.
Can F- cells initiate conjugation? Why or why not?
No, F- cells cannot initiate conjugation because they do not have an F pilus.
What is the tra region on the F plasmid responsible for?
The tra region contains genes required for conjugation, including those that create the F pilus.
What is the function of the oriT region on the F plasmid?
The oriT region serves as the origin of replication during conjugation.
Describe the first step in F plasmid conjugation.
The F pilus of the F+ donor cell binds to a receptor on the outer membrane of the F- recipient cell.
What happens after the F pilus binds to the F- cell?
The F pilus retracts, pulling the two cells closer together.
At what region is the F plasmid cut during conjugation?
The F plasmid is cut at the oriT region.
What is transferred from the F+ cell to the F- cell during conjugation?
A single strand of the F plasmid is transferred from the F+ cell to the F- cell.
How does the recipient cell become F+ after conjugation?
The single strand of the F plasmid is replicated in the recipient cell, forming a complete F plasmid and converting it to F+.
What is the outcome for both cells after F plasmid conjugation is complete?
Both the donor and recipient cells become F+ cells, each with a full copy of the F plasmid.
Why is the spread of the F plasmid significant in bacterial populations?
The spread of the F plasmid allows more cells to become F+ and capable of further conjugation, facilitating genetic exchange.