Ch. 14 - Biomedical Applications: Vaccines, Diagnostics, Therapeutics, and Molecular Method

Norman-McKay- Microbiology: Basic and Clinical Principles 2nd Edition

Norman-McKay2nd EditionMicrobiology: Basic and Clinical PrinciplesISBN: 9780137661619당신이 사용하는 게 아니라요?교과서 변경

Norman-McKay 2nd Edition

Ch. 14 - Biomedical Applications: Vaccines, Diagnostics, Therapeutics, and Molecular Method

문제 5

10장, 문제 5

A direct ELISA test is utilized as a pregnancy test because it tests for:
a. Antibodies to the fetus in urine
b. Antibodies to a pregnancy hormone in urine
c. An antigen that recognizes a pregnancy hormone in urine
d. T cells that recognize a pregnancy hormone in urine
e. The presence of a pregnancy hormone in urine

검증된 단계별 안내

Step 1: Understand what a direct ELISA test detects. A direct ELISA is designed to detect the presence of an antigen in a sample by using an antibody that binds directly to that antigen.

Step 2: Identify the target of a pregnancy test. Pregnancy tests typically detect the hormone human chorionic gonadotropin (hCG), which is present in urine during pregnancy.

Step 3: Recognize that in a direct ELISA pregnancy test, the test uses antibodies that specifically bind to the hCG hormone (the antigen) in the urine sample.

Step 4: Eliminate options that mention antibodies to the fetus, antibodies to a hormone, or T cells, since the test is not detecting immune cells or antibodies but rather the hormone itself as an antigen.

Step 5: Conclude that the correct understanding is that the direct ELISA test detects the presence of the pregnancy hormone (hCG) antigen in urine.

비슷한 문제에 대한 검증된 영상 답변:

이 영상 해법은 위 문제에 도움이 된다고 튜터들이 추천한 것입니다.

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주요 개념

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Direct ELISA Technique

Direct ELISA is an immunoassay that detects the presence of a specific antigen in a sample by using an enzyme-linked antibody that binds directly to that antigen. It is commonly used for identifying proteins or hormones, such as the pregnancy hormone hCG, without the need for secondary antibodies.

Pregnancy Hormone (hCG)

Human chorionic gonadotropin (hCG) is a hormone produced during pregnancy, detectable in urine and blood. Pregnancy tests rely on detecting hCG as a biomarker, since its presence indicates implantation and early pregnancy.

Antigen vs. Antibody Detection

In immunoassays, antigens are the target molecules being detected, while antibodies are the detection tools. Direct ELISA identifies antigens (like hCG) directly, whereas indirect ELISA detects antibodies. Understanding this distinction is key to interpreting test results.