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Ch. 18 - Practical Applications of Immunology
Tortora - Microbiology: An Introduction 14th Edition
Tortora14th EditionMicrobiology: An IntroductionISBN: 9780138200398당신이 사용하는 게 아니라요?교과서 변경
모든 교과서Tortora 14th EditionCh. 18 - Practical Applications of Immunology문제 5
14장, 문제 5

Use the following choices to answer questions 4 and 5:
a. Anti-Brucella
b. Brucella
c. Substrate for the enzyme
Which item is from the patient in an indirect ELISA test?

검증된 단계별 안내
1
Understand the principle of an indirect ELISA test: it is used to detect antibodies in a patient's sample by using a known antigen attached to a surface.
Identify the components involved in an indirect ELISA: the antigen (which is fixed on the plate), the patient's sample (which may contain antibodies), and a secondary antibody that binds to the patient's antibodies.
Recognize that in an indirect ELISA, the patient's sample provides the antibodies that will bind to the antigen coated on the plate.
From the given choices, determine which item represents the patient's contribution in the test. Since the patient provides antibodies, the correct choice corresponds to 'anti-Brucella' (antibodies against Brucella).
Conclude that the item from the patient in an indirect ELISA test is the antibody specific to the antigen used, which is 'anti-Brucella'.

비슷한 문제에 대한 검증된 영상 답변:

이 영상 해법은 위 문제에 도움이 된다고 튜터들이 추천한 것입니다.
영상 길이:
2m

주요 개념

질문에 올바르게 답하기 위해 반드시 이해해야 하는 핵심 개념들은 다음과 같습니다.

Indirect ELISA Test

Indirect ELISA is a laboratory technique used to detect antibodies in a patient's sample. It involves coating a plate with antigen, adding the patient's serum (which may contain antibodies), and then using a secondary antibody with an enzyme to produce a measurable signal.
추천 영상:
06:59
The Ames Test

Role of Antibodies in Indirect ELISA

In an indirect ELISA, the antibodies come from the patient’s sample and bind specifically to the antigen coated on the plate. Detecting these antibodies helps determine if the patient has been exposed to or infected by a particular pathogen.
추천 영상:
00:48
Antibodies

Components of ELISA Test Setup

Key components include the antigen (e.g., Brucella), the patient’s antibodies, and the enzyme substrate. The antigen is fixed on the plate, the patient’s antibodies bind if present, and the substrate reacts with the enzyme linked to the secondary antibody to produce a detectable signal.
추천 영상:
06:59
The Ames Test
관련 실천
교과서 질문

Label components of the direct and indirect ELISA tests in the following situations. Which test is direct? Which test provides definitive proof of disease?

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교과서 질문

The examples in questions 1 and 2 are:

a. Direct tests

b. Indirect tests

1554
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교과서 질문

How are monoclonal antibodies produced?

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교과서 질문

Explain the effects of excess antigen and antibody on the precipitation reaction. How is the precipitin ring test different from an immunodiffusion test?

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교과서 질문

In an immunodiffusion test, a strip of filter paper containing diphtheria antitoxin is placed on a solid culture medium. Then bacteria are streaked perpendicular to the filter paper. If the bacteria are toxigenic,

a. The filter paper will turn red

b. A line of antigen–antibody precipitate will form

c. The cells will lyse

d. The cells will fluoresce

e. None of the above

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교과서 질문

Use the following choices to answer questions 4 and 5:

a. Anti-Brucella

b. Brucella

c. Substrate for the enzyme

Which is the third step in a direct ELISA test?

1418
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