객관식
Which of the following statements about prions is true?
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1. A Review of General Chemistry
Intro to Organic Chemistry
객관식
Why is DNA polymerase commonly used in instead of other DNA polymerases?
A
It can synthesize DNA faster than other polymerases.
B
It is more accurate than other DNA polymerases.
C
It requires fewer primers for DNA synthesis.
D
It is resistant to the high temperatures used in .
검증된 단계별 안내1
Understand the purpose of PCR (Polymerase Chain Reaction): PCR is a technique used to amplify specific DNA sequences, making millions of copies of a particular DNA segment.
Recognize the conditions of PCR: The process involves repeated cycles of heating and cooling, which include denaturation (around 95°C), annealing (50-65°C), and extension (72°C).
Identify the role of DNA polymerase in PCR: DNA polymerase is an enzyme that synthesizes new DNA strands by adding nucleotides complementary to the template strand.
Consider the stability of enzymes at high temperatures: Most DNA polymerases would denature and lose their function at the high temperatures used during the denaturation step of PCR.
Learn about Taq DNA polymerase: Taq polymerase is derived from the thermophilic bacterium Thermus aquaticus, which thrives in hot environments. This makes Taq polymerase resistant to the high temperatures of PCR, allowing it to remain active throughout the cycles.
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