객관식
Which class of RNA molecules contains an anticodon?
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- 1. A Review of General Chemistry5h 6m
- Summary23m
- Intro to Organic Chemistry4m
- Atomic Structure17m
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- 20. Phenols55m
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- 23. The Chemistry of Thioesters, Phophate Ester and Phosphate Anhydrides1h 10m
- 24. Enolate Chemistry: Reactions at the Alpha-Carbon1h 50m
- Tautomerization9m
- Tautomers of Dicarbonyl Compounds6m
- Enolate4m
- Acid-Catalyzed Alpha-Halogentation4m
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- Haloform Reaction8m
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- Overview of Alpha-Alkylations and Acylations6m
- Enolate Alkylation and Acylation12m
- Enamine Alkylation and Acylation16m
- Beta-Dicarbonyl Synthesis Pathway7m
- Acetoacetic Ester Synthesis13m
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- 25. Condensation Chemistry2h 9m
- 26. Amines1h 43m
- 27. Heterocycles2h 0m
- Nomenclature of Heterocycles15m
- Acid-Base Properties of Nitrogen Heterocycles10m
- Reactions of Pyrrole, Furan, and Thiophene13m
- Directing Effects in Substituted Pyrroles, Furans, and Thiophenes16m
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- 28. Carbohydrates5h 54m
- Monosaccharide20m
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- 29. Amino Acids4h 16m
- Proteins and Amino Acids19m
- L and D Amino Acids14m
- Polar Amino Acids14m
- Amino Acid Chart1h 14m
- Acid-Base Properties of Amino Acids33m
- Isoelectric Point14m
- Amino Acid Synthesis: HVZ Method12m
- Synthesis of Amino Acids: Acetamidomalonic Ester Synthesis16m
- Synthesis of Amino Acids: N-Phthalimidomalonic Ester Synthesis13m
- Synthesis of Amino Acids: Strecker Synthesis13m
- Reactions of Amino Acids: Esterification7m
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- Reactions of Amino Acids: Ninhydrin Test11m
- 30. Peptides and Proteins2h 42m
- Peptides12m
- Primary Protein Structure4m
- Secondary Protein Structure17m
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- Disulfide Bonds17m
- Quaternary Protein Structure10m
- Summary of Protein Structure7m
- Intro to Peptide Sequencing2m
- Peptide Sequencing: Partial Hydrolysis25m
- Peptide Sequencing: Partial Hydrolysis with Cyanogen Bromide7m
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- Merrifield Solid-Phase Peptide Synthesis18m
- 31. Catalysis in Organic Reactions1h 30m
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- Intro to Citric Acid Cycle7m
- Structures of the Citric Acid Cycle19m
- The Citric Acid Cycle35m
- 34. Nucleic Acids1h 32m
- 35. Transition Metals6h 14m
- Electron Configuration of Elements45m
- Coordination Complexes20m
- Ligands24m
- Electron Counting10m
- The 18 and 16 Electron Rule13m
- Cross-Coupling General Reactions40m
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- Buchwald-Hartwig Amination Reaction19m
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- Catalytic Allylic Alkylation18m
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- 36. Synthetic Polymers1h 49m
- Introduction to Polymers6m
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- Copolymers6m
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- Polymers Structure and Properties8m
34. Nucleic Acids
Intro to Nucleic Acids
객관식
What is the function of a in sequencing?
A
To initiate strand synthesis
B
To terminate strand elongation
C
To enhance the fidelity of polymerase
D
To stabilize the double helix
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검증된 단계별 안내1
Understand the role of ddNTPs (dideoxynucleotides) in DNA sequencing. They are modified nucleotides that lack a 3'-OH group on the sugar moiety, which is crucial for forming phosphodiester bonds with the next nucleotide.
Recognize that in DNA sequencing, particularly Sanger sequencing, ddNTPs are used to terminate DNA strand elongation. This is because the absence of the 3'-OH group prevents the addition of further nucleotides.
Incorporate ddNTPs into the DNA synthesis reaction along with normal dNTPs (deoxynucleotides). The random incorporation of ddNTPs results in the termination of the DNA strand at various lengths.
Analyze the resulting DNA fragments, which are terminated at different points, to determine the sequence of the original DNA strand. Each fragment ends with a ddNTP, which can be identified by its fluorescent or radioactive label.
Conclude that the primary function of ddNTPs in DNA sequencing is to terminate DNA strand elongation, allowing for the determination of the DNA sequence by analyzing the lengths of terminated fragments.
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