Which of the following RNA sequences would be synthesized using the DNA template strand ?
A
B
C
D
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1
Identify the direction of the DNA template strand, which is given as 5'-CAGTTCGGA-3'. The RNA is synthesized in the 5' to 3' direction, so the template strand is read in the 3' to 5' direction.
Determine the complementary RNA bases for each DNA base. Remember that in RNA, adenine (A) pairs with uracil (U), thymine (T) pairs with adenine (A), cytosine (C) pairs with guanine (G), and guanine (G) pairs with cytosine (C).
Starting from the 3' end of the DNA template strand, write the complementary RNA sequence: DNA 3'-GGAACCTG-5' will pair with RNA 5'-CCUUGGAC-3'.
Reverse the RNA sequence to match the 5' to 3' direction: 5'-UCCGAACUG-3'.
Compare the synthesized RNA sequence with the given options to identify the correct one: 5'-UCCGAACUG-3'.