Viruses are very small (~20 nm), some protozoa are large (~5 mm), and bacteria are about 1 µm (0.001 mm).
Why study microbiology?
Microbes produce oxygen, fix nitrogen, recycle organic waste, are part of the food chain, produce food and pharmaceuticals, and play a role in human immunity.
Microbial role in environmental cleanup
Certain bacteria like Clostridium bifermentans degrade explosives like TNT, and Pseudomonas helps clean oil spills.
Microbes in food production
Yeasts and bacteria ferment cacao seeds to produce chocolate; microbes also produce beer, wine, pickles, and cheese.
Microbes in industry
Bacteria like Alcaligenes eutrophus can produce biodegradable plastics; enzymes from bacteria help soften denim fabric.
Microbes as biocontrol agents
Certain bacteria are used as insecticides to control crop diseases.
Robert Hooke's contribution to microbiology
Used a crude microscope to view cells, starting the cell theory that all living organisms are composed of cells.
Antoni van Leeuwenhoek's contribution
Built microscopes to view living microorganisms, which he called 'animalcules'.
Spontaneous generation vs Biogenesis
Spontaneous generation: life arises from non-living matter. Biogenesis: life arises only from pre-existing living matter.
Louis Pasteur's experiment
Used swan-necked flasks to disprove spontaneous generation and developed aseptic technique.
Pasteur's fermentation discovery
Yeasts convert sugar to alcohol anaerobically; bacteria convert alcohol to vinegar; pasteurization kills bacteria to prevent spoilage.
Germ theory of disease
Microbes cause chemical and physical changes and are responsible for diseases, replacing earlier beliefs of supernatural causes.
Joseph Lister's contribution
Used phenol to disinfect surgical instruments and wounds, reducing infections and leading to antiseptic techniques.
Robert Koch's postulates
Steps to prove a specific microbe causes a specific disease, demonstrated with anthrax in cows.
Louis Pasteur and vaccination
Weakened or killed microbes can induce immunity, demonstrated with fowl cholera.
Edward Jenner's vaccine
Developed smallpox vaccine using cowpox to induce immunity.
Paul Ehrlich and selective toxicity
Proposed chemicals could target microbes without harming the host; discovered salvarsan to treat syphilis.
Alexander Fleming's discovery
Discovered penicillin, the first antibiotic, produced naturally by mold to inhibit bacterial growth.
Microbial nomenclature
Genus and species names are Latinized, italicized or underlined; genus capitalized, species lowercase, e.g., Escherichia coli.