Q: If the diameter of the field of view is 4 mm at 4x objective, what will be the diameter of the field at 40x objective? Use the formula: $$\text{Diameter}_A \times \text{Magnification}_A = \text{Diameter}_B \times \text{Magnification}_B.$$

$$\frac{4 \times 4}{40} = 0.4 mm$$

Q: If the field of view at 10x objective is 2 mm, what is the field of view at 40x objective? Use the formula: $$\text{Diameter}_A \times \text{Magnification}_A = \text{Diameter}_B \times \text{Magnification}_B.$$

$$\frac{2 \times 10}{40} = 0.5 mm$$

Question 1

A student is using a compound microscope with a 10x ocular lens and a 40x objective lens. What is the total magnification being used?

Accepted Answer

$$10 	imes 40 = 400x$$

Question 2

Why is it important to start focusing on a slide using the lowest objective power (4x) before moving to higher powers?

Accepted Answer

It reduces the risk of damaging the slide and makes it easier to locate the specimen.

Question 3

If the diameter of the field of view is 4 mm at 4x objective, what will be the diameter of the field at 40x objective? Use the formula: $$	ext{Diameter}_A 	imes 	ext{Magnification}_A = 	ext{Diameter}_B 	imes 	ext{Magnification}_B.$$

Accepted Answer

$$\frac{4 	imes 4}{40} = 0.4 mm$$

Question 4

Which part of the compound microscope is responsible for concentrating light onto the specimen?

Accepted Answer

Condenser

Question 5

A student is having trouble distinguishing two closely spaced objects under high power. What adjustment should they make to improve resolution?

Accepted Answer

Increase the amount of light using the diaphragm lever.

Question 6

What is the function of the fine adjustment knob on a compound microscope?

Accepted Answer

To make small, precise focusing adjustments at high power.

Question 7

If you switch from a 10x objective to a 40x objective, what happens to the working distance?

Accepted Answer

It decreases.

Question 8

Which of the following best describes 'resolution' in microscopy?

Accepted Answer

The ability to distinguish two close objects as separate.

Question 9

A student observes that the image becomes dimmer as they increase magnification. What is the most likely reason for this?

Accepted Answer

The opening to the lens becomes smaller at higher powers, allowing less light through.

Question 10

If the total magnification is 100x and the ocular lens is 10x, what is the power of the objective lens being used?

Accepted Answer

$$\frac{100}{10} = 10x$$

Question 11

Which of the following is NOT a reason to use the fine adjustment knob only at high power?

Accepted Answer

It increases the total magnification.

Question 12

A student wants to increase the resolution at high power. Which of the following actions would be most effective?

Accepted Answer

Move the condenser closer to the stage.

Question 13

If the field of view at 10x objective is 2 mm, what is the field of view at 40x objective? Use the formula: $$	ext{Diameter}_A 	imes 	ext{Magnification}_A = 	ext{Diameter}_B 	imes 	ext{Magnification}_B.$$

Accepted Answer

$$\frac{2 	imes 10}{40} = 0.5 mm$$

Question 14

Which component of the microscope is adjusted to change the amount of light reaching the specimen?

Accepted Answer

Diaphragm lever

Question 15

A student is using a 100x objective lens with a 10x ocular lens. What is the total magnification?

Accepted Answer

$$10 	imes 100 = 1000x$$

Question 16

Which of the following best explains why a compound microscope is called 'compound'?

Accepted Answer

It uses two sets of lenses: objective and ocular.

Question 17

If a specimen is in focus under the 4x objective, what should you do before switching to the 40x objective?

Accepted Answer

Focus using the fine adjustment knob only.

Question 18

Which of the following is a correct statement about the relationship between magnification and field of view?

Accepted Answer

As magnification increases, the field of view decreases.

Question 19

A student is viewing a specimen at 400x total magnification and wants to estimate the size of a cell that takes up half the field of view. If the field of view at this magnification is 0.4 mm, what is the approximate size of the cell?

Accepted Answer

$$0.4 \div 2 = 0.2 mm$$

Question 20

Which of the following is a real-world consequence of not increasing light intensity at higher magnifications?

Accepted Answer

The image will appear dim and details may be lost, making diagnosis or analysis difficult.

Microscopy in Anatomy & Physiology: The Compound Microscope

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