Question 1

Which part of the microscope is responsible for concentrating light onto the specimen, and what is its correct position during use?

Accepted Answer

The condenser; it should be raised up close to the stage.

Question 2

If you are using a 40x objective lens and a 10x ocular lens, what is the total magnification of the specimen?

Accepted Answer

$$40 	imes 10 = 400$$

Question 3

A student is having trouble focusing on a specimen using the high power objective lens. Which adjustment knob should they use, and why?

Accepted Answer

Fine adjustment knob; it allows for small, precise changes in focus after coarse adjustment.

Question 4

When switching from the scanning objective lens to the high power objective lens, what should you do to maintain focus and avoid damaging the slide?

Accepted Answer

Use only the fine adjustment knob and avoid using the coarse adjustment knob.

Question 5

Which part of the microscope allows you to adjust the amount of light passing through the specimen?

Accepted Answer

Iris diaphragm

Question 6

A student observes that the image remains mostly in focus when switching between objective lenses. What property of the microscope does this demonstrate?

Accepted Answer

Parfocal

Question 7

If the field of view under the scanning objective lens is 4 mm, what will happen to the field of view when switching to the high power objective lens?

Accepted Answer

The field of view will decrease.

Question 8

Why is it important to start focusing with the scanning objective lens before moving to higher magnifications?

Accepted Answer

It provides a larger field of view and reduces the risk of damaging the slide.

Question 9

A student is using oil immersion objective lens. What must they do to use it correctly?

Accepted Answer

Place a drop of oil on the slide and ensure the lens tip extends into the oil.

Question 10

Which part of the microscope should you use to move the slide left and right or forward and backward for precise positioning?

Accepted Answer

Mechanical stage adjustment knobs

Question 11

If you want to increase the resolution of your microscope when viewing a specimen, which action is most effective?

Accepted Answer

Switch to a higher power objective lens.

Question 12

What is the function of the ocular lens in a compound microscope?

Accepted Answer

It magnifies the image produced by the objective lens, typically by 10x.

Question 13

A student notices that the pointer in the microscope is not centered in their field of view. What should they do?

Accepted Answer

Use the mechanical stage controls to reposition the slide.

Question 14

Which part of the microscope should never be used when focusing under high power or oil immersion objective lenses?

Accepted Answer

Coarse adjustment knob

Question 15

If the total magnification is calculated by multiplying the magnification of the objective lens by the ocular lens, what is the total magnification when using a 100x oil immersion objective and a 10x ocular lens?

Accepted Answer

$$100 	imes 10 = 1000$$

Question 16

What is the primary function of the revolving nosepiece on a microscope?

Accepted Answer

It allows the user to switch between different objective lenses.

Question 17

A student is viewing colored threads on a slide. Which concept is best demonstrated by observing which thread is in focus at different depths?

Accepted Answer

Depth of field

Question 18

Why is it important to use both eyes when viewing a specimen through the ocular lenses?

Accepted Answer

It reduces eye strain and allows for better depth perception.

Question 19

Which part of the microscope is used to turn the light source on and off?

Accepted Answer

Power switch

Question 20

A student is using the microscope and notices that the specimen is too dim at high magnification. What should they do?

Accepted Answer

Increase the light intensity using the light intensity knob.

Microscopy Lab: Structure and Use of the Compound Light Microscope

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