Question 1

Which part of the compound microscope is responsible for moving the slide in the X and Y directions to precisely position the specimen under the objective lens?

Accepted Answer

The stage adjustment knobs

Question 2

If you are using a 40X objective lens and a 10X ocular lens, what is the total magnification of the specimen you are viewing? Use the formula $	ext{Total Magnification} = 	ext{Objective Magnification} 	imes 	ext{Ocular Magnification}$.

Accepted Answer

$400	imes$

Question 3

A student is having trouble seeing the details of a stained cell under the microscope. Which adjustment is most likely to improve the contrast of the image?

Accepted Answer

Closing the iris diaphragm

Question 4

What is the primary function of the condenser lens in a compound microscope?

Accepted Answer

To focus light onto the specimen

Question 5

Why is immersion oil used with the 100X objective lens?

Accepted Answer

It reduces light refraction and increases resolution by matching the refractive index of glass.

Question 6

Which of the following best describes the difference between magnification and resolution in microscopy?

Accepted Answer

Magnification enlarges the image, while resolution distinguishes two points as separate.

Question 7

A student observes a specimen using a 10X ocular lens and a 4X objective lens. If the field of view at this setting is 4 mm, what will the field of view be if the student switches to a 40X objective lens, assuming the ocular lens remains the same? Use the formula $	ext{Field of View}_{	ext{new}} = \frac{	ext{Total Magnification}_{	ext{old}}}{	ext{Total Magnification}_{	ext{new}}} 	imes 	ext{Field of View}_{	ext{old}}$.

Accepted Answer

$0.4\ 	ext{mm}$

Question 8

When carrying a compound microscope, which two parts should you hold to ensure safe transport?

Accepted Answer

The arm and the base

Question 9

A student is viewing a specimen under high power and notices that the image is blurry. Which adjustment should be made to sharpen the focus without risking damage to the slide or lens?

Accepted Answer

Use the fine focus adjustment knob

Question 10

Which of the following statements about the path of light in a compound microscope is correct?

Accepted Answer

Light passes from the light source through the condenser, then the specimen, objective lens, and finally the ocular lens before reaching the eye.

Question 11

If a microscope's ocular lens is 10X and the objective lens is 100X, but the image appears dark and lacks detail, what is the most likely cause?

Accepted Answer

The oil immersion technique was not used, causing loss of resolution.

Question 12

Which of the following best explains why a fish appears closer to the surface when viewed from above water, as illustrated in Figure 1?

Accepted Answer

Light bends as it passes from water to air due to refraction.

Question 13

A student wants to increase the amount of detail seen in a transparent, unstained specimen. Which of the following would be the best first step?

Accepted Answer

Decrease the diameter of the iris diaphragm to increase contrast.

Question 14

Which of the following is NOT a function of the objective lens in a compound microscope?

Accepted Answer

Adjusting the amount of light passing through the specimen

Question 15

If the total magnification is 400X and the field of view is 0.5 mm, what would the field of view be at 100X total magnification? Use the formula $	ext{Field of View}_{	ext{new}} = \frac{	ext{Total Magnification}_{	ext{old}}}{	ext{Total Magnification}_{	ext{new}}} 	imes 	ext{Field of View}_{	ext{old}}$.

Accepted Answer

$2.0\ 	ext{mm}$

Question 16

Which of the following best describes the image produced by the ocular (eyepiece) lens in a compound microscope?

Accepted Answer

A virtual, magnified image

Question 17

A student is preparing a temporary wet mount slide. Which of the following is a critical step to avoid trapping air bubbles under the coverslip?

Accepted Answer

Lower the coverslip at an angle onto the drop of liquid.

Question 18

Which of the following is the best practice for cleaning the lenses of a microscope?

Accepted Answer

Use lens paper and lens cleaning solution.

Question 19

If you accidentally get immersion oil on a lens that is not the oil immersion lens, what should you do before continuing your observations?

Accepted Answer

Wipe the lens clean with lens paper before using it again.

Question 20

Which of the following best explains why increasing magnification decreases the field of view?

Accepted Answer

As magnification increases, the area visible through the lens decreases proportionally.

Microscopy: Principles, Structure, and Use in Anatomy & Physiology

Study Guide - Practice Questions

Study Guide - Flashcards