Question 1

Which part of the compound light microscope is responsible for adjusting the amount of light that reaches the specimen?

Accepted Answer

Diaphragm adjustment lever

Question 2

A student is using a microscope with a 10X ocular lens and a 40X objective lens. What is the total magnification?

Accepted Answer

$$10 	imes 40 = 400$$

Question 3

When switching from the 4X to the 40X objective lens, what happens to the working distance?

Accepted Answer

It decreases

Question 4

Which sequence correctly describes the order of microscope parts that light passes through from the light source to your eye?

Accepted Answer

Light source → condenser → stage aperture → objective lens → ocular lens

Question 5

A student is having trouble bringing a specimen into sharp focus under high power. Which adjustment should be used for fine focusing?

Accepted Answer

Fine focus knob

Question 6

If the image appears too dark under the microscope, which adjustment is most appropriate to increase brightness without changing focus?

Accepted Answer

Open the diaphragm adjustment lever

Question 7

Which part of the microscope is used to move the slide left, right, forward, or backward while viewing?

Accepted Answer

Mechanical stage adjustment knobs

Question 8

What is the primary function of the condenser in a compound light microscope?

Accepted Answer

To focus light onto the specimen

Question 9

A student observes that the field of view becomes smaller as they increase magnification. What is the best explanation for this observation?

Accepted Answer

Higher magnification lenses have a smaller field of view

Question 10

Which step should always be performed before switching to a higher power objective lens?

Accepted Answer

Center the specimen in the field of view

Question 11

If a microscope slide is not visible when looking through the ocular lenses, what is the first adjustment you should check?

Accepted Answer

Objective lens is clicked into place

Question 12

Which lens should you use first when beginning to focus on a new specimen?

Accepted Answer

4X scanning objective

Question 13

A student wants to calculate the total magnification using a 10X ocular lens and a 100X oil immersion objective. Which formula and answer are correct?

Accepted Answer

$$10 	imes 100 = 1000$$

Question 14

Which of the following best describes the function of the coarse focus knob?

Accepted Answer

It makes large adjustments to the stage height for initial focusing

Question 15

Why is it important to start with the lowest power objective when viewing a new slide?

Accepted Answer

It provides the largest field of view, making it easier to locate the specimen

Question 16

Which part of the microscope should you hold when carrying it to ensure safety?

Accepted Answer

Arm and base

Question 17

A student is using the 100X oil immersion objective but the image is blurry. What is the most likely cause?

Accepted Answer

Oil was not applied between the lens and the slide

Question 18

Which action should be taken before storing the microscope after use?

Accepted Answer

Lower the stage and rotate the 4X objective into position

Question 19

If the image is clear in one ocular lens but blurry in the other, what adjustment should be made?

Accepted Answer

Adjust the interpupillary distance and diopter

Question 20

Which of the following is NOT a function of the mechanical stage adjustment knobs?

Accepted Answer

Focusing the image

Microscopy: Structure, Function, and Use in Anatomy & Physiology

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