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Which of the following properties is NOT typically exploited in protein purification?
Why is it important to follow the sequential order of techniques in a protein purification strategy?
Which technique would you use immediately after protein extraction in a typical purification strategy?
Which of the following is NOT a component of the crude extract?
What is a major challenge in obtaining purified proteins from the crude extract?
Which of the following best describes a challenge in purifying proteins from a crude extract?
Which of the following factors does NOT affect the sedimentation coefficient of a particle?
What is the rationale for the order of separation of cell components during differential centrifugation?
Two proteins have the same mass but different sedimentation coefficients. What could explain this difference?
Which process allows proteins to transition from insoluble precipitates to a dissolved state?
Why is ammonium sulfate commonly used in the salting out process?
What additional techniques might be needed after salting out for complete protein purification?
How does a semipermeable membrane function in the dialysis process?
Why is dialysis not effective for separating proteins from other proteins?
What is a limitation of dialysis in protein purification, and what subsequent step can be taken?