What is a limitation of dialysis in protein purification, and what subsequent step can be taken?

• A. Dialysis increases salt concentration; subsequent step is filtration.
• B. Dialysis denatures proteins; subsequent step is gel electrophoresis.
• C. Dialysis cannot separate proteins from other proteins; subsequent step is chromatography.
• D. Dialysis cannot remove salts; subsequent step is centrifugation.

Answer: (C)