A mixture of proteins and nucleic acids is analyzed. How can the absorbance spectrum be used to determine the relative concentrations of these biomolecules?

• A. By measuring absorbance only at 280 nm.
• B. By comparing absorbance at 260 nm and 280 nm.
• C. By measuring absorbance only at 260 nm.
• D. By using a single wavelength for all biomolecules.

Answer: (B)