What role do the dissociation rate constant (k-ESI) and association rate constant (kESI) play in determining the inhibition constant (K'I) for the enzyme-substrate-inhibitor complex?

• A. They determine the ratio that defines the inhibition constant (K'I).
• B. They determine the substrate concentration required for half-maximal inhibition.
• C. They determine the enzyme concentration required for complete inhibition.
• D. They determine the maximum reaction velocity of the enzyme-substrate-inhibitor complex.

Answer: (A)