How does Le Chatelier's principle explain the effect of uncompetitive inhibitors on enzyme affinity?

• A. The decrease in enzyme-substrate complex concentration shifts the equilibrium to the right, increasing enzyme affinity for the substrate.
• B. The equilibrium shifts to the left, increasing enzyme affinity for the substrate.
• C. The equilibrium remains unchanged, so enzyme affinity is unaffected.
• D. The increase in enzyme-substrate complex concentration shifts the equilibrium to the left, decreasing enzyme affinity for the substrate.

Answer: (A)