Which sequence of steps correctly illustrates the process of collecting and purifying proteins using column chromatography?

• A. Elute proteins, load protein mixture, add mobile phase, collect purified proteins.
• B. Add mobile phase, load protein mixture, elute proteins, collect purified proteins.
• C. Collect purified proteins, load protein mixture, add mobile phase, elute proteins.
• D. Load protein mixture, add mobile phase, elute proteins, collect purified proteins.

Answer: (D)