If the concentration of an inhibitor is 5 mM and the inhibition constant K_I is 2 mM, what is the degree of inhibition (α) on the free enzyme?

Analyze the role of α' in enzyme inhibition when the inhibitor concentration is 10 mM and K'_I is 5 mM. What is α' and what does it signify?

What happens to enzyme activity when an inhibitor binds to form EI and ESI complexes?

In a drug development scenario, how would you use the degree of inhibition to assess the effectiveness of a new enzyme inhibitor?

Synthesize how the degree of inhibition (α and α') modifies the Lineweaver-Burk equation in the presence of mixed inhibitors.

What does a degree of inhibition (α) greater than 1 indicate?

What is the relationship between inhibitor concentration and the degree of inhibition (α) for the free enzyme?