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What would be the consequence of a missing 3 prime hydroxyl group during DNA synthesis?
Which of the following is a limitation of the dideoxy DNA sequencing method compared to modern techniques?
In a dideoxy sequencing experiment, what would be the result of using equal concentrations of dNTPs and ddNTPs?
What is the primary reason dideoxynucleotide triphosphates are used in DNA sequencing?
What is the rationale for conducting separate reactions for each dideoxynucleotide triphosphate in dideoxy sequencing?
Why are pyrophosphate and hydrogen ions important byproducts in DNA synthesis?
How does DNA polymerase utilize deoxynucleotide triphosphates during DNA synthesis?