In a multi-step enzyme-catalyzed reaction, how does the rate-limiting step influence kcat?

• A. The rate-limiting step determines the value of kcat, which limits the maximum reaction rate.
• B. The rate-limiting step has no effect on kcat.
• C. The rate-limiting step increases the overall reaction rate by increasing kcat.
• D. The rate-limiting step only affects the binding affinity of the enzyme.

Answer: (A)