A mutation in phospholipase C (PLC) prevents it from hydrolyzing PIP2. What would be the expected consequence in the signaling pathway?

• A. Increased production of cAMP.
• B. Increased activation of adenylate cyclase.
• C. Enhanced activation of protein kinase A.
• D. No production of IP3 and DAG, leading to reduced calcium release and PKC activation.

Answer: (D)