How do you determine the x-intercept on a Lineweaver-Burk plot, and what does it signify?

• A. It is the reciprocal of Vmax, indicating the maximum reaction velocity.
• B. It is the slope of the line, indicating the Km/Vmax ratio.
• C. It is the negative reciprocal of Km, indicating the inverse of substrate concentration.
• D. It is the point where the line crosses the y-axis, indicating the initial velocity.

Answer: (C)