Biochemistry
What is the primary difference between size exclusion chromatography and gel electrophoresis in terms of molecular movement?
How do the porous beads in the stationary phase of size exclusion chromatography affect the elution of proteins?
If a chromatogram shows three peaks with elution times of 5, 10, and 15 minutes, which peak corresponds to the largest protein?
Which of the following mixtures would be best separated using size exclusion chromatography?
How would you estimate the size of an unknown protein using size exclusion chromatography?
How can you use size exclusion chromatography to determine the size of an unknown protein in a mixture?
What does a longer elution time on a chromatogram indicate about a protein's size?
